多功能植病生防木霉分离筛选和鉴定.docVIP

2009 杨合同，于雪云，魏艳丽，周红姿，宋莉璐，扈进冬，李纪顺。多功能植病生防木霉的分离筛选与鉴定。山东科学，22（5）：27－30 多功能植病生防木霉的分离筛选与鉴定基金项目：国家“863” 基金项目：国家“863”计划现代农业技术领域重大项目,编号2006AA10A211 通讯作者：杨合同，男，博士，硕士生导师，Tel:0531，E-mail:yanghet@ 杨合同,于雪云,魏艳丽,周红姿,宋莉璐,扈进冬,李纪顺 (山东省科学院中日友好生物技术研究中心,山东济南250014 ) 摘要 从山东省临沂、聊城、寿光、章丘四个地区的西红柿、黄瓜大棚，韭菜、白菜菜地，大蒜、大葱种植区采取的120份土壤样品中分离获得木霉244株。对峙培养发现其中对棉花立枯丝核菌具有拮抗能力的有114株；固体培养检测木霉的解磷和固氮能力，发现所有菌株都能够水解植酸钙，有74株能够水解磷酸钙，未发现能在无氮培养基上生长的木霉菌株。对抑菌圈和解磷圈最大的19株木霉在液体培养条件下检测了解磷和解钾能力，其中菌株T13-8（1.792μg/mL）、T23(1.652μg/mL)显示出较高的解磷能力，部分菌株在液体培养条件下不能解磷；部分菌株在液体培养下显示出解钾功能，其中菌株T47-4(20.585μg/mL)显示出较高的解钾能力。上述19个菌株的形态学和ITS序列分析鉴定表明，其中9个菌株为Trichoderma longibrachiatum，8个菌株为T. harzianum，另外2个菌株分别为T. virens和T. citrinoviride。 关键词：多功能；木霉；解磷；植酸酶；解钾 Abstract Isolation, screening and identification of multifunctional Trichoderma spp. with biocontrol capacity against plant pathogens A number of 244 Trichoderma was isolated from 120 soil samples collected from greenhouse field of tomato, cucumber, Chinese chive, Chinese cabbage, garlic and green onions from Linyi , Liaocheng, Shouguang, Zhangqiu of Shandong Province. All of the 244 Trichoderma strains were dually cultured with Rhizoctonia solani which is a pathogen to cotton, and 114 isolates were shown to inhibit Rhizoctonia solani, of which 19 isolatesshowed better inhibitory ability than the others. All of the 244 Trichoderma strains were detected in their capability to degrade insoluble-phosphate, insoluble-potassium and fix nitrogen by solid plate culture method. The result showed that 74 isolates have obvious insoluble-phosphate solubilization ability, the above mentioned 19 isolates which were better in inhibitory ability showed also the best solubilization ability. The result also showed that no Trichoderma strain solubilize insoluble- potassium, , and there is no isolates could grow on nitrogen-free medium. The 19 Trichoderma isolates with stronger inhibitory and insoluble-phosphate solubilization ability were detected in their ability to solubilize insoluble-ph