草莓致腐霉菌的鉴定及其产毒能力研究-食品安全质量检测学报.PDF

8 5 Vol. 8 No. 5 2017 5 Journal of Food Safety and Quality May. , 2017 魏 超 1, 2*, 代晓航 1, 2, 郭灵安 1, 2, 雷欣宇 1 (1. , 610066; 2. (), 610066) 摘 要: 目的 , 方法 , ITS ; A(ochratoxin A, OTA)结果 4 1 , OTA, OAT 结论 , , , 关键词: ; ; A Identification and toxin-producing capability of causing-spoilage fungi in strawberry WEI Chao1, 2*, DAI Xiao-Hang1, 2, GUO Ling-An1, 2, LEI Xin-Yu1 (1. Analysis and Testing Center of Sichuan Academy of Agricultural Sciences , Chengdu 610066, China; 2. Security Risk Assessment Laboratory of Ministry of Agriculture (Chengdu), Chengdu 610066, China) ABSTRACT: Objective To investigate the main causing-spoilage fungi on the surface of strawberries and analyze whether it generates toxins. Methods The fungi were isolated from strawberries. Taxonomic status of fungi was conducted by colony morphology observation, reproduction microscopic observation and ITS gene sequence phylogenetic analysis. High performance liquid chromatography (HCLP) was used to detect the content of ochratoxin A (OTA) in spoilage strawberries and fungal fermentation broth. Results Four kinds of Mucor and one Penicillium could cause strawberry decay, which were identified as Mucor fragilis , Mucor circinelloides , Mucor racemosus, Rhizomucor variabilis and Penicillium sp. at molecular level. OTA could not be detected form spoilage strawberries and Mu