水引发对油松种子和幼苗的生理效应及其作用机理.PDFVIP

第 35 卷第 21期 生 态 学 报 Vol.35,No.21 2015年11月 ACTA ECOLOGICA SINICA Nov.,2015 DOI:10.5846/ stxb201401200147 贺红娟,管桦,张存莉.水引发对油松种子和幼苗的生理效应及其作用机理.生态学报,2015,35(21):7033鄄7042. He HJ,Guan H,Zhang C L.Physiological effects and mechanisms of Pinus tabulaeformis seeds and seedlings by hydro鄄priming.Acta Ecologica Sinica, 2015,35(21):7033鄄7042. 水引发对油松种子和幼苗的生理效应及其作用机理 贺红娟,管摇 桦,张存莉* 西北农林科技大学生命科学学院,杨凌摇 712100 摘要:为了解决油松种子在直播造林中发芽率低、幼苗抗旱性差的问题,研究了油松种子水引发的方法及引发对其种子和幼苗 的生理效应,并对水引发后幼苗的抗旱机理进行了探索。 结果表明:油松种子水引发的最佳条件为10 益,闭光条件下分批给种 子加水,加水总量为种子初始质量的30%,引发 10 d。 水引发可显著(P0.05)降低种子的半数发芽天数和硬实率,显著提高种 子的发芽指数、最终发芽率和活力指数;水引发种子的可溶性糖及可溶性蛋白含量相比对照也得到提高。 水引发可增加油松幼 苗的茎粗、鲜重及根长,降低其相对苗高;并极显著(P0.01)提高了幼苗过氧化氢酶、过氧化物酶和根内脱氢酶活性,降低了游 离氨基酸含量。 干旱胁迫下,引发种子的幼苗脯氨酸和可溶性糖含量分别高于对照 39.6%、118.9%,丙二醛含量低于对照 93.4%,均达极显著水平。 综合分析认为,水引发提高了种子可溶性糖及可溶性蛋白含量为种子萌发提供了保障,从而提高了 种子发芽率,并通过增强幼苗抗氧化酶活性、降低丙二醛含量,进而增强幼苗抗氧化系统的功能,最终明显提高其抗旱性。 关键词:油松;水引发;生理效应;种子萌发;干旱胁迫 Physiological effects and mechanisms of Pinus tabulaeformis seeds and seedlings by hydro鄄priming HE Hongjuan,GUAN Hua,ZHANG Cunli* College of Life Sciences,NorthwestAgriculture and Forestry University,Yangling 712100,China Abstract:The hydro鄄priming techniquewas adoptedforPinus tabulaeformis seedsto solvethe poor emergence and drought resistance of direct seeding in the present study. The effect of hydro鄄priming on the seed, seedling, and the drought resistance mechanism of the primed seedling were also explored. The results showed that the optimal condition for hydro鄄 priming was at 10 毅Cfor 10 days under dark condition,and in the presence of 30% additional water to the original seed mass. Specifically,a succession of total water percentages (53.6%,14.5%,14.5%,8.3%,5.7%,and 3.4%)was added at 0 h, 8 h, 16 h, 40 h, 64 h, and 88 h after starting priming, respectively. The effects of hydro鄄priming on P. tabulaeformis seeds