HPLC 测定小儿清咽颗粒中靛蓝和靛玉红的含量 - 现代仪器与医疗.PDF

检验检测 2013 年 第 19 卷 第 4 期 HPLC测定小儿清咽颗粒中靛蓝和靛玉红的含量 Determination of Indigo and Indirubin in Xiaoer Qingyan Keli by HPLC 摘  要  目的 采用高效液相色谱法(HPLC ）分析小儿清咽颗粒中有效成分靛蓝和 靛玉红的含量。方法 以Agilent HC-C18(250 mm ×4.6 mm,5 mm) 色谱柱, 以乙腈-0.1% 醋酸溶液(78:22 ）作为流动相, 流速为1.0 mL/min, 柱温:30℃；检测波长:292 nm。 结果 靛蓝和靛玉红分别在0.009 ～0.180mg/mL 和0.64 ～12.80mg/mL 范围内有良 好的线性关系, 平均加样回收率(n=6) 分别为98.3%、97.2%,RSD(n=6) 分别为1.26%、 胡艳红* 李志浩   0.83%。结论 本方法准确, 简便, 重复性好, 可用于小儿清咽颗粒中靛蓝和靛玉红的 Hu Yanhong Li Zhihao 定量分析。 关键词  小儿清咽颗粒 靛蓝 靛玉红 高效液相色谱法 (湖北医药学院附属东风医院药 学部 十堰 442008) Abstract Objective To establish the RP-HPLC method for the determination of indigo (Department of Pharmacy Dongfeng an indirubin in Xiaoer Qingyan Keli . Methods The Agilent HC-C18 (250 mm×4.6 mm,5 Hospital Affiliated to Hubei Medical mm) column was used, the mobile phase consisted of acetonitrile: 0.1% HAc(78:22) , the University Shiyan 442008) flow rate was 1.0mL/min, the column temperature was 30 ℃ the detecting wavelength was at 292 nm. Results The calibration curve was linear within a range of 0.009～0.180mg/ mL and 0.64～12.80mg/mL, the average recovery of this method(n=6) was 98.3%, 97.2% and the RSD(n=6) was 1.26%, 0.83%, respectively. Conclusion The method is simple, repeatable and accurate, it can be applied in quantitative determination of indigo and indirubin in Xiaoer Qingyan Keli. Key words Xiaoer Qingyan Keli Indigo Indirubin HPLC 中图分类号:R284.1    文献标识码:B    文章编号:2055-