常用生化检测项目分析方法举例及参数设置(Methods of analysis of biochemical tests are used for example and parameter setting).docVIP
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常用生化检测项目分析方法举例及参数设置(Methods of analysis of biochemical tests are used for example and parameter setting)
Methods of analysis of biochemical tests are used for example and parameter setting
The method of analysis of biochemical test is recommended
1. The finish is commonly used method to detect a total bilirubin (oxidation or diazo method), in combination with bilirubin (oxidation or diazo method), serum total protein (biuret method) (bromocresol chloride method), serum albumin, total bile acid (enzymatic), glucose (glucose oxidase method), uric acid (uric acid enzymatic), total cholesterol (cholesterol oxidase method), triglycerides (glycerol phosphate oxidase enzymatic), high-density lipoprotein cholesterol (direct measurement), calcium (azo arsenic Ⅲ method), p (uv), magnesium (dimethyl aniline blue method), etc. The above projects, in addition to calcium, phosphorus and magnesium basically still use single reagent method analysis and by using a bit of the finish, determination of other projects can use double reagent can choose two end point method, including the determination of total protein and albumin were already double reagents available.
2. Fixed time method for determination of creatinine by acid method.
3. Continuous monitoring for enzyme activity determination, generally should choose continuous monitoring method, such as alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, alkaline phosphatase, gamma pancreatic ammonia acyltransferase, amylase and creatine kinase, etc. A number of metabolic enzymes, such as hexokinase determination of glucose and urease coupling, can also be used for continuous monitoring.
4. Transmission turbidimetry transmission turbidimetric method can be used for the determination of turbidity response project, most belong to immune turbidimetry, apolipoprotein, immunoglobulin and complement, fight O, rheumatoid factor, as well as in the serum before other proteins such as albumin, transferrin, haptoglobin a
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