磷酸根的鉴定（Identification of phosphate）.doc

磷酸根的鉴定（Identification of phosphate） BaCl2 or FeCl3 with phosphate solution reaction, generate white precipitate barium phosphate and white precipitation of iron phosphate content was calculated according to the amount of phosphate precipitation. 3Ba2++2PO3- = Ba3 (PO4): 2 Fe3+ + PO4 = FePO4: 3- 1. determination of orthophosphate content (1) method summary Under acidic conditions, orthophosphate reacts with ammonium molybdate to form a yellow phosphorus molybdenum heteropoly acid, which is then reduced to ascorbic acid blue by ascorbic acid. The maximum absorption wavelength at 710nm is determined by spectrophotometry. (2) reagents and materials A. potassium dihydrogen phosphate; B. sulfuric acid solution (1+1); C. ascorbic acid solution (20g/L): 10g ascorbic acid, accurate to 0.5g, weighing two 0.2g sodium ethylenediaminetetraacetic acid (C10H14O8N2Na2.2H2O), accurate to 0.01g, dissolved in 200mL water, add 8.0mL acid, dilute with water to 500mL, mixing, stored in a brown bottle (valid for one Months); D. ammonium molybdate solution (26g/L): 13g ammonium molybdate, accurate to 0.5g, weigh 0.5g of potassium antimony tartrate (KSbOC4H4O6.1/2H2O), accurate to 0.01g, dissolved in 200mL water, adding 230mL sulfate (1+1) solution, mixing, cooling after diluted with water to 500mL, mixing, and stored in a brown bottle (valid for two months); E. P stock standard solution (1mL containing 0.5mgPO43-): weigh accurately 0.7165g advance in drying at 100~105 Deg. C and has a constant weight of potassium dihydrogen phosphate, accurate to 0.0002g, soluble in water is about 500mL, the quantitative transfer to a 1L volumetric flask, dilute with water to the scale, shake; F. phosphorus standard solution (1mL containing 0.02mgPO43-): take 20.00mL phosphorus standard storage solution in 500mL capacity bottle, dilute it to the scale and shake it well. (3) instruments and equipment Spectrophotometer: with a thickness of 1 cm of the absorption cell. (4) analysis procedure Draw A. curve: ta