用大孔吸附树脂分离川芎总提物（Separation of total extract of Ligusticum chuanxiong Hort with macroporous adsorption resin）.doc

用大孔吸附树脂分离川芎总提物（Separation of total extract of Ligusticum chuanxiong Hort with macroporous adsorption resin） Separation of total extract of Ligusticum chuanxiong Hort with macroporous adsorption resin Update Date: 2009-07-29 Click: Deng Shaowei, Ma Shuangcheng The Chuanxiong reflux extraction with alcohol reduced concentration, macroporous adsorption resin column, and washed with water, eluting with 30% ethanol, 30% ethanol eluates were concentrated to extract the total alkaloid of Rhizoma Chuanxiong, ligustrazine and ferulic acid content of about 25% to more than 29% of the product, the yield is 0.6%. Keywords Rhizoma Chuanxiong; total extract; extraction and separation; macroporous resin; Ligusticum chuanxiong is the rhizome of Ligusticum, wallichii, Franch. of, an umbrella plant. The chemical constituents of Rhizoma Chuanxiong is very complex, mainly containing special odor volatile oil of ferulic acid, alkaloids, phenolic substances, volatile oil and neutral substances, delta gamma, alpha and beta unsaturated lactone. In addition, it also contains vitamin A, folic acid, sterols, sucrose, fatty acids and so on. Reported that total alkaloids and phenolic constituents of Rhizoma Chuanxiong have obvious dilation of coronary artery, increase coronary blood flow, reduce myocardial oxygen consumption, inhibit ADP induced platelet aggregation, have effect on the aggregation of platelet depolymerization has reduced platelet surface activity, the pharmacological effects of 2. The content of ligustrazine in the rhizome of Ligusticum chuanxiong is 0.01% ~ 0.02%, and the content of ferulic acid in Ligusticum chuanxiong Hort is about 0.02% [2]. The extraction methods of Ligustrazine and ferulic acid were mostly extracted by ethanol, separated by acid and alkali treatment and column chromatography, but not suitable for industrial production. The author has done a great deal of research work on the extraction of total alkaloids and phenolic compounds in extracts, in order to obt