quantity one的使用方法（How to use quantity one）.docVIP

quantity one的使用方法（How to use quantity one） Execution step: Because Quantiy One can only identify the black-and-white TIF files, but in fact many published papers, magazines are required to provide TIF format images and non JEPG format; therefore, considering the two factors, I suggest you from the beginning to scan and unify the storage as TIF format. 1. Lane creation: A. opens the electrophoresis picture we want to analyze (take protein electrophoresis as an example). B. selects Lane-Auto Frame lanes. If you compare the electrophoresis images standard, Quantity One will automatically identify each track position, and the path of each lane with a red line marked out; If you are not satisfied with the automatic labeling on software lane, you can provide the Lane-Edit Frame subordinate sub menu functions of lane frame location and size adjustment; adjustment method is through the realization of the mouse to drag and drop. If you only need to analyze several markers lane data without interference to other lanes of your sight, you can remove the mark you dont need the lane through the Lane-Single Lane-Remove Lane. Be careful： Not all of the pictures can be electrophoresis lanes of Quantity One automatic identification. Not in the automatic identification of the case, Quantity One will tell you that it does not recognize the pop-up dialog box lane. Then you need to manually draw track. The method is the same as above, with the Lane-Single Lane-Creat Lane to achieve. As long as with the mouse in the electrophoresis images on your drop axis to be marked down the lane can draw the lane mark line. 2. different lanes to exclude background: We will conduct our electrophoresis image Lane mentioned above, whether automatic or manual recognition. Then select the Lane-Lane Backgroud... command. Select this command, the mouse becomes a green +, the mouse you want to exclude background lane, click the left button, immediately pops up the dialog box shown below. The Optical Density d