MIT机电工程与计算机科学系【本科生课程】6.07J.生命科学中的微型工程.Projects.in.Microscale.Engineering.for.the.Life.Sciences[www.ed2kers.com].pdfVIP

Projects in Microscale Engineering for the Life Sciences As taught in: Spring 2007 Members of the class inspect one of the wafers they created in lab. (Image courtesy of A. Aranyosi.) Instructors: Prof. Dennis Freeman Prof. Martha Gray Prof. Alexander Aranyosi MIT Course Number: HST.410J / 6.07J Level: Undergraduate Course Description This course is a project-based introduction to manipulating and characterizing cells and biological molecules using microfabricated tools. It is designed for first year undergraduate students. In the first half of the term, students perform laboratory exercises designed to introduce (1) the design, manufacture, and use of microfluidic channels, (2) techniques for sorting and manipulating cells and biomolecules, and (3) making quantitative measurements using optical detection and fluorescent labeling. In the second half of the term, students work in small groups to design and test a microfluidic device to solve a real-world problem of their choosing. Includes exercises in written and oral communication and team building. Syllabus Course Meeting Times Lectures: 2 sessions / week, 3 hours / session Contents - Engineering Systems at the Scale of Cells and Biomolecules  How do you build tools that can manipulate cells? o Lithography: Shrinking patterns to the micro-scale o Soft lithography: Making bio-compatible fluid reservoirs o Rapid prototyping: Using existing microscale structures to test new ideas  How do you measure properties of individual cells? o Optical imaging o Electrical signals o Cell sorting o Averaging Teaching/Learning Activities  Two lectures each week to introduce new material.  Two lab sessions each week to provide hands-on experience.  One project to help students learn to pose testable hypotheses, to