一、工程菌的构建和表达 - 第三军医大学学报.DOCVIP

鲍曼不动杆菌噬菌体AB3裂解酶基因的表达及其抗菌活性分析 张劼，周丽蓉，罗永艾（重庆医科大学附属第一医院呼吸科，重庆 400016）鲍曼不动杆菌以ET28a(＋为载体构建重组质粒，在大肠杆菌表达，金属离子合亲和层析法纯化重组蛋白Expression and antibacterial activity assay of bacteriophage AB3 endolysin against Acinetobacter baumannii Zhang Jie , Zhou lirong , Luo Yongai（Department of Respiratory Medicine, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China） [Abstract] Objective To construct the prokaryotic expression vector of the bacteriophage AB3 endolysin (LysAB3), and investigate the antibacterial activity of LysAB3 against Acinetobacter baumannii. Methods The LysAB3