青黛炮制过程中靛蓝定向生成的工艺设计与优化-天津中草药杂志社.PDF

• 56 • 中草药 Chinese Traditional and Herbal Drugs 第 42 卷 第 1 期 2011 年 1 月 青黛炮制过程中靛蓝定向生成的工艺设计与优化 1 1 1, 2* 3 刘泽玉 ，苏柘僮 ，杨 明 ，邹文铨 1. 成都中医药大学，四川 成都 610075 2. 江西中医学院 中药现代制剂教育部重点实验室，江西 南昌 330004 3. 四川大学，四川 成都 610065 摘 要：目的 筛选出青黛炮制过程中显著影响靛蓝生成的因素，优化最佳水平组合，确定生成靛蓝的最佳工艺。方法 应 用 HPLC 法测定粗靛中靛蓝；并以鲜叶产靛蓝的量（即靛蓝的转移率）为指标，采用 Plackett-Burman 设计，Box-Behnken 设计效应面分析等统计学方法，筛选出显著影响因素及最佳水平组合。结果 青黛的浸泡、打靛工艺确定为浸泡前不除蜡， 浸泡液 pH 3.5 ，溶剂用量与鲜叶质量比例为 13，避光、浸泡24 h ，温度25 ℃，通气时间 30 min，加氨水调节打靛前pH 值 至 9 。结论 系统地优化了青黛浸泡、打靛环节的工艺，阐明了青黛炮制过程中各个影响因素对有效成分靛蓝的影响，为炮 制原理的研究奠定了基础。 关键词：青黛；靛蓝；Plackett-Burman 设计；Box-Behnken 设计；最佳工艺 中图分类号：R284.2; R286.02 文献标识码：A 文章编号：0253 - 2670(2011)01 - 0056 - 05 Design and optimization of technology for orientation generated indigo in processing of Indigo Naturalis 1 1 1, 2 3 LIU Ze-yu , SU Zhe-tong , YANG Ming , ZOU Wen-quan 1. Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China 2. Key Laboratory of Modern Preparation of Chinese Materia Medica, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China 3. Sichuan University, Chengdu 610065, China Abstracts: Objective Screening the influencing factors that affect the indigo-generated significantly in processing of Indigo Naturalis, the levels were optimized and combined to obtain the optimum technology for the indigo-generated. Methods Using an HPLC method for determining indigo in crude Indigo Naturalis and taking indigo content generated from fresh leaf as an index, the significantly influencing factors and the optimal level combination were screened by the statistical methods of Plackett-Burman design and Box-Behnken design response surface analysis. Results The soaking and making indigo proce