当归藤水提物及醇提物急性毒性实验探究.docVIP

当归藤水提物及醇提物急性毒性实验探究[摘要] 目的 对当归藤水提取物和醇提取物的急性毒性进行初步研究，评价其安全性，为新药开发提供理论依据。 方法 采用最大给药量测定法，小鼠灌胃给药测定当归藤水提取物和醇提物的半数致死量（median lethal dose，LD50）或最大耐受量（maximum tolerated dose，MTD），研究其安全性。 结果 当归藤水提取物和醇提取物的毒性都很小，未测得灌胃给药小鼠半数致死量LD50，水提取物和醇提取物的MTD 90 g/kg，最大耐受量倍数相当成人（50 kg）用量的150倍。 结论 当归藤无明显毒性，临床常用量安全可行。 [关键词] 当归藤；急性毒性实验；最大耐受量 [中图分类号] R284.1 [文献标识码] A [文章编号] 1673-7210（2012）07（a）-0029-02 Experimental study of acute toxicity of water extract and ethanol extract of Angelica vine LU Senhua CHEN Yong ZENG Haisheng ZHANG Long ZHENG Xiren ZHOU Yuan College of Pharmacy, Guangxi University of Traditional Chinese Medicine, Guangxi Zhuang Autonomous Region, Nanning 530001, China [Abstract] Objective To study the acute toxicity of water extract and ethanol extract of Angelica vine, evaluate their safety and provide theoretical basis for the development of new drugs. Methods The maximum administration determination method was used and the drug was administered orally. The median lethal dose (LD50) and maximum tolerated dose (MTD) of water extract and ethanol extract of Angelica vine were measured to study the safety. Results The toxicity of water extract and alcohol extract of Angelica vine was very small, thus the LD50 of mice could not be obtained. MTD of water extract and ethanol extract was larger than 90 g/kg. The maximum tolerated dose multiple equals 150 times of the dose for adults (50 kg). Conclusion Angelica vine does not have apparent toxicity, thus its clinical routine dose is safe and feasible. [Key words] Angelica vine; Acute toxicity experiment; Maximum tolerated dose 当归藤为紫金牛科酸藤子属植物当归藤（Embelia parviflora Wall. ex A. DC.）的根与老茎，别名小花酸藤子、筛箕蔃、土当归等。其味苦、涩，性温，具有补血、活血、益精壮阳、强腰膝的功效。常用于治疗血虚诸症、月经不调、闭经、产后虚弱、腰腿酸痛、跌打骨折等症[1-2]，在广西少数民族地区常用它作当归使用，故民间流传有“谁人懂得筛箕蔃，不愁不生养”之说，说明该药可治疗不孕症等[3]。当归藤还有温肾补血作用，广西特产中成药“桂龙药膏”以该药材为原料。陈家源等[4]从该药材的根茎乙醇提取物中分离得到4个结晶，其中3个鉴定为正三十烷酸乙酯、正三十烷酸及α-菠甾醇，另1个结晶初步分析为具侧链的羟基苯醌类成分。杨瑞云、管海波等[5-6]用紫外分光光度法对当归藤中总黄酮提取工艺进行研究及儿茶素含量的测定。经查阅文献目前尚未见该药材急性毒性实验研究的报道。本文对当归藤的水提物和醇提物进行