辣椒疫病生防菌株鉴定及控病机理研究-中国农学通报.PDF

中国农学通报 2012,28(10): 136-141 Chinese Agricultural Science Bulletin 辣椒疫病生防菌株鉴定及控病机理研究 1 2 2 2 2 2 2 马晓飞 ，刘长远 ，赵奎华 ，梁春浩 ，关天舒 ，王 辉 ，李柏宏 1 2 （沈阳农业大学植物保护学院，沈阳110866；辽宁省农业科学院植物保护研究所，沈阳110161） 摘 要：为筛选出对辣椒疫病菌具有较强抑制活性的生防菌株，达到控制辣椒疫病的目的，从辣椒种植区 土壤中分离大量微生物，采用平板对峙法筛选对辣椒疫病菌具有较强抑制作用的生防菌株XD6 ，通过 常规方法和16SrDNA 确定其分类地位，并测定相关酶活性变化。试验分离得到的XD6 菌株，平板对峙 试验防治效果高达80%~100% ，初步鉴定为多粘芽孢杆菌(Paenibacillus p olymyxa) 。酶活测定显示，生 防菌株XD6 的β-1,3-葡聚糖酶和纤维素酶活性都存在显著变化，且接种XD6 菌株的辣椒植株PAL 活性、 PPO 活性、POD 活性均明显升高。菌株XD6 对辣椒疫病具有较高防效，其生防因子可作用于辣椒疫病 菌细胞壁，并诱导辣椒自身防御酶系的增强，具有良好的研究前景。 关键词：辣椒疫病；筛选；生防菌株；鉴定；控病机理 中图分类号：S4 文献标志码：A 论文编号：2011-3174 Study on Identification and Control Mechanism of Biocontrol Phytophthora capsici 1 2 2 2 2 2 2 Ma Xiaofei , Liu Changyuan , Zhao Kuihua , Liang Chunhao , Guan Tianshu , Wang Hui , Li Baihong 1 ( College of Plant Protection, Shenyang Agricultural University, Shenyang 110866; 2Institute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang 110161) Abstract: The biocontrol strains were screened which had strong inhibitory activity against Phytophthora capsici, in order to control pepper plague. A large number of microorganisms were isolated from soil of pepper growing areas, and a bacterium named XD6 which having strong antagonistic effect on Phytophthora capsici by plate confrontation was obtained. Through methods of traditional and 16S rDNA, the status of category was identified, and then the cha