Identification of the Volatile Constituents of （的挥发性成分的识别）.pdf

Leffingwell Reports, Vol. 5(2), 1-29, November 2013 Identification of the Volatile Constituents of Cyprian Latakia Tobacco by Dynamic and Static Headspace Analyses by 1 2 1 3 John C. Leffingwell , E. D. Alford , Diane Leffingwell and Roger Penn 1 Leffingwell Associates, Canton, GA 30115 USA 2 Alford Consulting, Louisville, KY 40207 USA 3 Mane SA, Route de lIndustrie 21, Vouvry 1896, Switzerland Summary The volatile constituents of Cyprian Latakia tobacco were determined by both dynamic and static headspace GC-MS analyses. In excess of 500 volatile constituents were identified, with the majority of volatiles being phenolics, polycyclic and related aromatic hydrocarbons, sesquiterpenoids and known tobacco constituents. Notably, five ambrettolide isomers and dihydroambrettolide, as well as (Z)-Octadec-9-en-18-olide, were identified. Previous work: No comprehensive analysis of the volatile constituents of Latakia tobacco has been previously published. In the late 1960’s, publications by Irvine Saxby reported identification of 38 phenols (1,2) and 22 carboxylic acids (3) of Latakia tobacco . Another study by these authors led to the identification of 27 anilines and aliphatic amines (4). In 1972, Elmenhorst identified the methyl ethyl cyclopentenolones (5) and in 1980 found 28 alkylnaphthalenes in Latakia tobacco (6). The most extensive previous work on Latakia tobacco was carried out by Tolman at British American Tobacco during 1982-1983 in a series of unpublished reports (7-9). Introduction: Latakia tobacco is a minor tobacco type produced by subjecting the leaf of Oriental tobacco to the smoke from “controlled fires of aromatic woods and fragrant herbs”. During the process, of “smoke-curing” the tobacco acquires a black color and peculiar tarry-smoky exotic aroma and flavor. Latakia tobacco w