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Lab 3. Microscopic Observation of Unicellular (实验室3。)
Lab 3: Microscope and Cells (revised fall 2009)
Lab 3. Microscopic Observation of Unicellular and Multicellular Organisms
Prelab Assignment
Before coming to lab, read carefully the introduction (pp. 1 – 6) and the procedures (pp. 6 – 17) for
each part of the experiment, and then answer the prelab questions at the end of this lab handout. Hand
in the prelab assignment just before the start of your scheduled lab period.
Goals of this Lab Exercise
After completing this lab exercise you should be able to...
Identify the parts of a compound light microscope and use a microscope to competently examine
biological samples.
Use a microscope to identify the following organisms or tissues: Amoeba, Trichonympha,
Protococcus, Scenedesmus, Volvox, Spirogyra, and human cheek epithelium
Accurately sketch, describe and cite the major functions of the structures and organelles of the cells
examined in this lab exercise.
Determine the diameter of the field of view for the various objectives of a microscope.
Estimate the size of specimens viewed with a microscope.
Describe the evidence that supports the theory that over time organisms have evolved from unicellular
organisms, to loose aggregates, to simple colonial organisms, to complex colonial organisms, and,
finally, to multicellular organisms.
Describe the similarities and differences in the structures of the animal and plant cells observed in this
lab exercise.
Introduction
The Microscope
The microscope is one of the principal tools of the biologist. Without the microscope, many of the
great discoveries of biology would never have been made. The light compound microscope, illustrated in
Figure 1 on the following page, is the type of microscope most commonly used. Proper, comfortable use
of t
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