基于CPLD工作模式可调的线阵CCD驱动电路设计-光子学报.PDF

490 2007, Vol. 28, No. 07 食品科学 ※营养卫生 宫智勇，王耀峰，刘良忠 (武汉工业学院食品科技学院，湖北 武汉 430023) ：目的：对食品污染物苯并[a]芘所致细胞损伤作用进行研究。方法：用不同浓度的苯并[a]芘(1、5、10、 50、100μmol/L)对肺腺癌细胞A549 染毒24h，MTT 法测定细胞存活率、单细胞琼脂糖凝胶电泳检测细胞DNA 损 伤，用溶剂二甲基亚砜处理细胞组作为未染毒细胞组。结果：苯并[a]芘染毒作用时，随染毒浓度的增加，A549 细胞存活率呈下降趋势；A549 细胞的DNA 损伤程度也随染毒浓度增加而增加，与未染毒A549 细胞相比，染毒 A549细胞的Olive尾矩值均有明显增加(p＜0.05)。结论：食品污染物苯并[a]芘致A549细胞明显的损伤作用。 ：食品污染物；苯并[a]芘；细胞损伤 Study on Damage of Cells Caused by Food Pollutant Benzo[a] Pyrene GONG Zhi-yong，WANG Yao-feng，LIU Liang-zhong (School of Food Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China) Abstract ：Objective: To investigate whether food pollutant benzo(a)pyrene causes the damage of A549 cells. Methods: A549 cells were exposed to different concentrations of benzo(a)pyrene (1, 5, 10, 50, 100μmol/L BaP) for 24h. We measured the ability of viable cells by MTT assay, and detected DNA damage with alkaline single-cell gel electrophoresis. Results: We found A549 cells, which exposed to 1, 5, 10, 50, 100μmol/L BaP, had a significant decreased growth rate compared with the group not exposed to BaP (p＜0.05). And we found the dose-dependent increases in DNA damage in A549 cells exposed to BaP. The OTM values in A549 cells exposed to 1, 5, 10, 50, 100μmol/L BaP all increased significantly compared with the group not exposed to BaP, suggested food pollutant benzo [a] pyrene could cause the damage of A549 cells. respectively (p＜0.05). Conclusion: It is Key words：food pollutant；benzo(a)pyrene；cell damage 中图分类号：R994.4 文献标识码：A 文章编号：1002-6630(2007)07-0490-03 多环芳烃是有机物不完全燃烧时产生的挥发性碳氢 1.1 仪器与试剂 化合物，是重要的食品和环境污染物，