正交试验优化L_色氨酸发酵培养基的研究.pdf

, Journal of AnhuiA gri. Sc.i 2011, 39( 4): 1910- 1911, 1914 乔利利 况玲玲 L 1, 2 2 2 2 2 王东阳 , 蔡传康 , 闫汝东 ,冯志彬 , 张华 ( 1. , 300222; 2. , 272000) [目的]优化 L色氨酸发酵培养基[方法]以大肠杆 TRJH 0709为供试 株, 通过单因素和正交试验研究了L色氨酸合成的最 佳发酵培养基[结果] L色氨酸最适发酵培养基为葡萄糖 50. 0 g/L硫酸铵 15. 0 g/L酵母粉 1. 5 g/L和柠檬酸 2. 0 g/L其中葡萄糖 对试验效果影响最大在该最优条件下, L色氨酸摇瓶产量可达 19. 0 g/L[结论]为 L色氨酸的中试及工业化生产提供了理论依据 L色氨酸;正交试验;培养基优化 TQ 922 A 0517- 6611( 2011) 04- 01910- 02 Optimization of Ltryptophan FermentationMedium byOrthogonalExperiment WANG Dongyang et al ( College of iotechnology, Tianjin University of Science Technology, Tianjin 300222) Abstract [Objective] The ami was to optmi izeLtryptophan ferm entation m edium. [Method] Themedium ofE. coli TRJH0709 for the pro duction of Ltryptophanw as optmi ized by single factor expermi ent and orthogonal designm ethod. [Result] Them ain factor affecting the produ cing Ltryptophanwasglucose among the orthogonal conditions. The optmi um ferm entation medium of Ltryptophanw asglucose 50. 0 g/L, am monium sulfate 15. 0 g /L, yeast extract 1. 5 g/L and sodium citrate 2. 0 g/L. Under the optmi al condition, Ltryptophan production reached 19. 0 g/L. [ Conclusion] The research provides theoreticalbasis for the pilot test and industrialproduction of Ltryptophan. ey words Ltryptophan; Orthogonal expermi ent; M edium optmi ization L 8 SO ∀ 7H O 0. 1 g/L, (NH ) SO 20. 0 g/L, pH 7. 0~ 7. 2, 4 2 4 2 4 , , 115 # 15m in [ 1] , 1. 3 , , L 1. 3. 1 1 , L 400m l 5 L, 9, ,, ( 200 r/m in), 32 # 8 h L, 1. 3. 2 10% ,,