参芝发酵产物的抗肿瘤活性及其对小鼠免疫功能的影响.PDFVIP

菌物学报 jwxt@ 15 November 2013, 32(6): 1056-1063 Http:// Mycosystema ISSN1672-6472 CN11-5180/Q © 2013 IMCAS, all rights reserved. 参芝发酵产物的抗肿瘤活性及其对小鼠免疫功能的影响 * 林琳 包海鹰 吉林农业大学中药材学院 吉林 长春 130118 摘 要：为了探索红参水煎液的灵芝发酵产物对H22 荷瘤小鼠的抗肿瘤活性及其对小鼠免疫功能的影响，通过体内 抗肿瘤实验和增强免疫功能实验从抑瘤率、对免疫器官的影响指数、对非特异性免疫、体液免疫及细胞免疫的影响5 个方面对该产物做了功能性评价。结果表明，在抗肿瘤实验中，参芝发酵产物高剂量组的抑瘤率达到51.65%，脾指 数和胸腺指数均高于对照组和环磷酰胺（CTX ）组；增强免疫功能实验中，3 个实验的给药组小鼠和对照组小鼠相比 都有显著性差异（P ＜0.01 ）。由此可见，将灵芝与人参在发酵层次上配伍具有显著的抑制荷瘤小鼠肿瘤生长及增强小 鼠免疫功能的作用。 关键词：灵芝，红参水煎液，液体发酵，抑瘤率，免疫增强作用 Anti-tumor activities and immunopotentiation to mice of Ganoderma lucidum-fermented product of ginseng-simmered extract * LIN Lin BAO Hai-Ying College of Chinese Traditional Medicine, Jilin Agricultural University, Changchun, Jilin 130118, China Abstract: Anti-tumor activities against H22 tumor and the immunopotentiation to mice of Ganoderma lucidum-fermented product of ginseng-simmered extract were evaluated in five aspects, viz. inhibition rates of tumor, immune organ indexes, nonspecific immunity, the humoral immunity and the cellular immunity. The inhibition rates of the fermentation products for high dosage treatment group reached 51.65%. The spleen indexes and thymus indexes were higher than those of the control group and cyclophosphamide-treated group (CTX). In the immunological enhancement experiment, the medicine treatment groups had significant difference as compared with the control groups (P0.01). These results indicated that Ganoderma lucidum-fermented product of ginseng-simmered extract could suppress tumor growth and had potent enhancement effect on the immune function of mice significantly. Key words: Ganoderma lucidum, ginseng water solution, liquid fermentation, anti-tumor rate, immunopotentiation 基金项目：人参的灵芝发酵保健饮料的中试及扩大生产工艺研究（No.