核桃楸皮甲醇提取物急性毒理探究.docVIP

核桃楸皮甲醇提取物急性毒理探究[摘要] 目的 测定中药核桃楸皮甲醇提取物小鼠半数致死量（LD50）。 方法 40只小鼠随机分为4组，每组10只，雌雄各半，以最大浓度最大容量灌胃给药，24 h内给药，观察7 d内小鼠的毒性反应及死亡情况，记录各组小鼠症状，以小鼠死亡率为指标，计算其LD50。 结果 核桃楸皮甲醇提取物的LD50为2.622 mg/kg，其95%可信范围为1.811 mg/kg ≤ LD50 ≤ 5.433 mg/kg。 结论 核桃楸皮甲醇提取物有明显的急性毒理反应。 [关键词] 核桃楸皮；半数致死量；小鼠；灌胃 [中图分类号] R965 [文献标识码] A [文章编号] 1673-7210（2012）01（b）-113-02 The acute toxicological study on Methanol extracts of Cortex Juglandis Mandshuricae LEI Tao LIANG Qichao LIN Feng WU Yiyan▲ School of Pharmacy, Mudanjiang Medical College, Heilongjiang Province, Mudanjiang 157011, China [Abstract] Objective To measure the half lethal dose of Methanol extracts of Cortex Juglandis Mandshuricae in mice. Methods Totally 40 mice in half genders were randomly divided into 4 groups, each had 10 mice with male and female half. Different doses of liquor of Cortex Juglandis Mandshuricae were feed to the mice within 24 hours, the toxicity effect and death situation of mice within seven days were observed, the symptoms to mice were recorded. Then the LD50 of Cortex Juglandis Mandshuricae was calculated according to the index of mice mortality rate. Results The LD50 of Methanol extracts of Cortex Juglandis Mandshuricae was 2.622 mg/kg and its 95％ confident limit was 1.811-5.433 mg/kg． Conclusion Methanol extracts of Cortex Juglandis Mandshuricae has obvious toxicity reaction. [Key words] Cortex Juglandis Mandshuricae; LD50; Mice; Intragastric administration 核桃楸皮（Cortex Juglandis Mandshuricae）为胡桃科核桃属植物核桃楸（Juglans mandshurica Maxim.）的树皮[1]。原植物核桃楸又名山核桃、胡桃、胡桃楸、小核桃等，为胡桃科胡桃属落叶乔木，主要分布于我国东北地区（小兴安岭及张广才岭），河北、北京、内蒙古、山西等地有少量分布，俄罗斯远东地区、朝鲜、日本也有分布[2]，其树皮及未成熟果实或果皮可用药，树皮又名楸树皮，味苦性寒，具有清热解毒、止痢明目的功效，主治泄泻、痢疾、白带、目赤[3]。现代研究表明，核桃楸具有抗氧化[4]、抗菌、消炎、抗癌等作用[5]。近几年核桃楸皮化学成分的分离提取和抗癌作用机制研究成为热点，经过现代对胡桃楸化学成分分析证明胡桃楸含有萘醌、胡桃醌、黄铜苷、鞣花酸及没食子酸等化学物质[6]。现为观察其甲醇提取液的毒性及安全性，进行急性毒性的实验研究。直接用SPSS软件计算LD50，具有简单、快速、直观和方便的特点[7]。 1 仪器与试药 核桃楸皮（核桃揪皮采自黑龙江省牡丹江市牡丹峰）；灌胃器、烧杯、量筒、旋转蒸发仪、电子天平等。 昆明种小鼠（牡丹江医学院实验动物中心提供），体重18～22 g，雌雄各半。实验前动物置于室内适应环境1周，室温20～28℃，采用架式笼养，标