缬沙坦及氟伐他汀降低阿霉素肾病大鼠蛋白尿及肾组织NF-κB表达实验探究.docVIP

缬沙坦及氟伐他汀降低阿霉素肾病大鼠蛋白尿及肾组织NF-κB表达实验探究[摘要] 目的 探讨缬沙坦与氟伐他汀对阿霉素（ADR）肾病大鼠蛋白尿及肾组织核因子κB（NF-κB）表达的影响。方法 雄性SD大鼠120只，适应性喂养2周后，随机抽取18只为正常对照组(A组)；另外102只制作阿霉素肾病模型。84只造模成功大鼠随机分为4组：B组为模型对照组（等容积生理盐水， n=21），C组为缬沙坦治疗组[缬沙坦3.5mg/（kgd），n=21]，D组为氟伐他汀治疗组[氟伐他汀10mg/（kgd），n=21]，E组为缬沙坦与氟伐他汀联合治疗组[缬沙坦3.5mg/（kgd） 加氟伐他汀10mg/（kgd），n=21]。其后，分别于2、6、10周末，遵循随机化原则，按n=6分层抽取各组样本，收集24h尿液、血液及肾组织标本待测。结果 与A组相比,B组、C组、D组和E组24h尿蛋白排泄、血清总胆固醇（TC）、甘油三酯（TG）浓度明显升高（P＜0.01）；免疫组化染色表明， NF-κB阳性积分明显升高（P＜0.01）；缬沙坦与氟伐他汀单用及联用尿蛋白排泄减少，血清TC、TG浓度降低（P＜0.05 或 P＜0.01），肾脏NF-κB表达降低（P＜0.05 或 P＜0.01）。结论 缬沙坦与氟伐他汀可减轻阿霉素肾病大鼠蛋白尿、降低血清TG、TC浓度，降低肾脏NF-κB表达，联用时疗效更明显。提示缬沙坦与氟伐他汀至少部分通过降低NF-κB表达而减轻肾脏损害。 [关键词] 阿霉素肾病；缬沙坦；氟伐他汀；核因子κB [中图分类号] R587.2[文献标识码] A[文章编号]1673-9701（2011）21-06-03 Effect of Valsartan and Fluvastatin on Reducting the Expression of Proteinuria and Nuclear Factor-κB (NF-κB) in Adriamycin-induced Nephrotic Rats ZHANG DaoyouWU XiaodongCUI MingchunYANG YanlangYANG LicaiZHU Xinjian. Departnent of Nephrology, Affiliated Yijishan Hospital,Wannan Medical College,Wuhu 241001,China [Abstract] Objective To investigate the influence effct of valsartan and fluvastatin on reducting the expression of proteinuria and nuclear factor -κB（NF-κB）in adriamycin-induced nephrotic rats. Methods Male Sprague-Dawley（SD）rats were randomly separated into five groups and given different therapies. Normal control group（group A,n=18）. Nephrotic model was induced by tail introvenously injection of ADR（6.0mg/kg）. Eighty-four ADR-induced nephrotic male SD rats were randomly divided into 4 groups: model control group(group B: normal saline 3mL/d,n=21), valsartan treatment group(group C, valsartan 3.5mg/（kg?d）, n=21）,fluvastatin treatment group(group D, fluvastatin 10mg/（kgd）, n=21） and combining valsartan with fluvastatin treatment group(group E,valsartan 3.5mg/（kgd）plus fluvastatin 10mg/（kgd）, n=21）. After the end of therapies for 2, 6, and 10 weeks, the samples of 24h urine , serum were collected and assayed (six rats were assigned randomly in every