胃肠道癌肿患者外周血CD4+CD25+FOXP3+调节性T细胞表达及临床意义.docVIP

胃肠道癌肿患者外周血CD4+CD25+FOXP3+调节性T细胞表达及临床意义【摘要】目的:研究胃肠道癌肿患者外周血CD4+CD25+FOXP3+调节性T(Treg)细胞的表达,并探讨其临床意义。方法:通过免疫荧光术及流式细胞仪检测20例胃癌患者及20例结肠癌患者外周血CD4+CD25+FOXP3+Treg细胞、CD4+CD25+high?Treg细胞、CD4+T细胞及CD4+CTLA-4+T细胞。结果:胃癌组、结肠癌组与健康献血者比较外周血CD4+CD25+FOXP3+Treg细胞、CD4+CD25+high?Treg细胞及CD4+CTLA-4+T细胞显著增多,CD4+T细胞显著减少;胃癌、结肠癌患者之间其外周血中CD4+CD25+FOXP3+Treg细胞、CD4+CD25+high?Treg细胞、CD4+T细胞及CD4+CTLA-4+T细胞无显著差异。结论:胃肠道癌肿患者外周血CD4+CD25+FOXP3+Treg细胞显著高于健康献血者,这可能与胃肠道癌肿患者的免疫抑制和肿瘤的进展相关。 【关键词】胃癌 结肠癌;CD4+CD25+FOXP3+Treg;CD4+;CD25+high?Treg CD4+T;CD4+CTLA-4+T Peripheral regulatory CD4+CD25+FOXP3+ T cells in patients with gastrointestinal m alignancies Chou Jun-Lan,Zhang Hui,Zhao Feng-lin,Cao Fei,Wu Huilian ,FENG Lin (Municipal Hospital, Suzhou, Jiangsu, Suzhou,215000) 【Abstract】 Objects: To demonstrate the possible involvement of CD4+CD25+FOXP3+ ?regulatory T(Treg)cells in immune system impairment in patients with gastrotestinal m alignancies. METHODS: The CD4+CD25+FOXP3+Treg cells、CD4+CD25+high?Treg cells、CD4+Tcells and CD4+CTLA-4+T cellswere analyzed in peripheral blood in 20 patients with gastric cancer,20 patients with colon cancer by flow and immunofluorescence. RESULTS: Compared with healthy controls, patients with gastric cancer and colon cancer had higher percentage of CD4+CD25+FOXP3+Treg cells、CD4+CD25+high?Treg cells and CD4+CTLA-4+T cells,had lower percentage of CD4+Tcells.There were no significant differences between healthy volunteers and patients with gastric cancer or colon cancer. CONCLUSIONS: The increased percent of CD4+CD25+FOXP3+Treg cells may be related to immunosuppression and tumor progression in patients with gastrointestinal m alignancies. 【Keywords】CD4+;CD25+high?Treg CD4+T;CD4+CTLA-4+T 在我国,相对身体其它系统而言,消化道癌肿的发病率居于首位。CD4+CD25+Treg细胞来源于胸腺,是免疫系统维持免疫稳态的重要组成成分,具有免疫无能性和免疫抑制性两大功能特点,通过直接的效应细胞之间的相互接触方式抑制存在于正常机体内潜在的自身反应性T细胞的活化与增殖,其在肿瘤免疫及自身免疫的调节中起重要作用。本研究采用流式细胞仪测定了胃、结肠癌患者的外周血中CD4+CD25+FOXP3+Treg细胞、CD4+CD25+high?Treg细胞、CD4+T细胞、和CD4+CTLA-4+T细胞,以探讨其临床意义。 1 材料与方法 1.1材料研究对