药用植物刺山柑悬浮培养细胞聚集体理化性质探究.docVIP

药用植物刺山柑悬浮培养细胞聚集体理化性质探究[摘要] 目的：探讨不同直径刺山柑细胞聚集体黄酮含量与其颗粒大小、生长状态的关系及可能机制。方法：根据细胞直径的不同，分拣出3种不同大小的刺山柑细胞聚集体，对它们进行了黄酮含量、相关酶活性及再培养曲线的测定。结果：不同的细胞聚集体在形态大小、黄酮含量、酶的活性、生长速度等方面均存在着差异，其中颗粒最小的细胞聚集体黄酮含量约为颗粒较大的细胞聚集体的2倍，并且其丙二醛含量、苯丙氨酸解氨酶及过氧化物酶的活性及再培养曲线均具有相同的变化趋势。结论：不同聚集体黄酮含量与其颗粒大小及生长状态有一定的关系，个体较小的与氧气、培养基养分接触充分，利于细胞生长与次生代谢物的积累有关。 [关键词] 刺山柑；悬浮培养；聚集体；黄酮；直径 [中图分类号] R961[文献标识码]A [文章编号]1673-7210（2008）07（c）-019-04 The research for the physico-chemical property of Capparis Spinosa L. suspension cell aggregation LIU Wei1, LI Mao-teng1*, ZHU Yu-mei2, GAN Lu1, YU Long-jiang1 (1.College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074,China; 2. Modern Education Center, Shandong Medical College, Linyi 276000,China) [Abstract] Objective:To investigate the relations between flavone content and particle size, growth condition of different diameters of Capparis Spinosa L. cell aggregation and discuss the possible mechanism. Methods:According to the differences of cell diameter, we selected 3 different sizes of Capparis Spinosa L. cell aggregation and determined flavone content, correlated enzyme activities and recultivate curve. Results:The cell aggregations had differences in shape as well as size, flavone content, enzyme activities, growth speed and so forth. The flavone content of the smallest cell aggregation was approximately twice compared to the larger ones, and its malonaldehyde content, the phenylalanine ammonialyase and peroxide enzyme activities and recultivate curve presented the same change tendency.Conclusion:The flavone content has certain relations with particle size and growth condition of different cell aggregation. The small individual contacts fully with the oxygen and the nutrient of culture medium, which is benefit to cell growth and the secondary metabolite accumulation. [Key words] Capparis Spinosa L.; Suspension culture; Aggregation; Flavone; Diameter 细胞聚集体是液体悬浮培养的植物细胞特有存在方式[1，2]。在研究中，往往将细胞培养物当作均匀的细胞或细胞聚集体而忽略了不同细胞