载脂蛋白B-载脂蛋白E及血管紧张素原基因多态性及冠心病关系.docVIP

载脂蛋白B\载脂蛋白E及血管紧张素原基因多态性及冠心病关系[摘要] 目的 探讨载脂蛋白B、E（ApoB、ApoE）基因及血管紧张素原基因（AGT）多态性与冠心病（CHD）的相关性。方法 采用基因芯片技术分析89例CHD患者和78例非CHD患者的ApoB Xba I、ApoE112/158及AGT M235T基因多态性及等位基因频率。结果 CHD组ApoB Xba I、ApoE112/158及AGT M235T基因型分布与对照组相比差异有统计学意义（P＜0.05）。CHD组的ApoB Xba I的X+X+基因型及等位基因X+频率显著高于对照组，差异有统计学意义（P＜0.05），ApoE112/158的ε3/4、ε4/4基因型及等位基因ε4频率高于对照组，差异有统计学意义（P＜0.05）；CHD组AGT-TT基因型频率及T等位基因频率均明显高于对照组，差异有统计学意义（P＜0.05）。结论ApoB Xba I、ApoE112/158和AGT M235T的基因多态性可能是中国人CHD的危险因素。ApoB Xba I等位基因X+；ApoE112/158的等位基因ε4和AGT M235T基因的T等位基因是CHD的重要遗传标记。 [关键词]冠心病；载脂蛋白B；载脂蛋白E；血管紧张素原；基因；多态性 [中图分类号] R541.4 [文献标识码] A [文章编号] 1673-9701（2011）19-04-03 The Research of Apolipoprtein B，E and Angiotensinogen Gene Polymorophisms in Predisposition to CHD MA Juan ZHANG Hongqing WANG Wei TIAN Qing Department of Cardiology，The First People’s Hospital of Kunming City，Kunmimg 650011，China [Abstract] Objective To explore the relationship of apolipoprotein B，E（ApoB，ApoE）and angiotensinogen（AGT）gene polymorphism with coronary heart disease（CHD）. Methods Gene chip technology was performed to analyze ApoB XbaⅠ、ApoE 112/158 and angiotensinogen gene polymorphism. The allele frequency and gene type distribution were compared between groups. ResultsThere was a significant difference in the distributuion of Apo B，ApoE and AGT gene type between patients and controls（P＜0.05）. The frequency of X+X+ genetype and X+ allele of ApoB were significantly higher in patients with CHD than controls（P＜0.05）；the frequency ofε3/4、ε4/4 genetype andε4 allele of ApoE112/158 were higher in patients with CHD than controls（P＜0.05）. AGT-TT gene type was related with CHD significantly（P＝0.003）and T allele was more frequent significantly in CHD group than controlls（P＜0.05）. ConclusionThe Apo B，ApoE and AGT gene polymorphism may be the risk factors of CHD；X+ allele of ApoB Xba Ⅰ andε4 allele of ApoE gene and T allele of AGT gene might be the geneticmarkers of CHD. [Key words] Coronary heart disease；Apolipoprotein B；Apolipoprotein E；Angiotensi