款冬花不同提取物对豚鼠离体回肠收缩作用的研究.PDFVIP

款冬花不同提取物对豚鼠离体回肠收缩作用的研究 王金凤 1 2 2 ，杨苏蓓 ，毛培江 (1.浙江中医药大学，杭州 310053 ；2.浙江省中药研究所有限公司，杭州 310023) 摘要：目的 研究比较款冬花各个不同提取物对豚鼠离体回肠收缩的作用。方法 通过石油醚、乙酸乙酯、CO2 超临界 萃取等方法制备款冬花多个提取物，将离体豚鼠回肠悬吊于克-亨试液中，观察给药后豚鼠回肠的收缩作用，计算豚鼠回 肠收缩幅度抑制率。结果 款冬花各个不同提取物均能降低组胺引起的豚鼠离体回肠收缩幅度。结论 款冬花各个提取 物能明显抑制豚鼠回肠收缩运动。 关键词：款冬花；豚鼠离体回肠；组胺 中图分类号：R285.5 文献标志码：A 文章编号：1007-7693(2010)09-0781-04 Study the Effects of Different Extracts of Tussilago Farara L. on the Contraction of Isolated Ileum of Guinea Pig 1 2 2 WANG Jinfeng , YANG Subei , MAO Peijiang (1.Zhejiang Chinese Medical University, Hangzhou 310053, China; 2.Zhejiang Research Institute of Traditional Chinese Medicine Co. Ltd, Hangzhou 310023, China) ABSTRACT: OBJECTIVE To investigate and compare the effect of different extracts of Tussilago farara L. on the contraction of isolated ileum of guinea pig. METHODS By the method of petroleum, acetoacetate and supercritical CO2 , the Tussilago Farara L. was extracted Isolated ileum of guinea pig was put in K-H solution. The contraction extent of isolated ileum was observed. Inhibition ratio of the contraction extent was calculated. RESULTS Different extracts of Tussilago Farara L. decreased the contraction extent of isolated ileum of guinea pig cased by histamine. CONCLUSION The extracts of Tussilago Farara L. significantly inhibited the contraction of isolated ileum of guinea pig . KEY WORDS: Tussilago Farara L; isolated guinea pig ileum; histamine 款冬花(Tussilago Farara L.)为菊科植物款冬的 年版收载，款冬花性温，味辛、微苦，具有润肺下 干燥花蕾，又名冬花、九九花等。中国药典 2005 气，止咳化痰等功能，用于新久咳嗽，喘咳痰多， 作者简介：王金凤，女，硕士 Te