表5部分辐射株系试管苗与一年生苗薯蓣皂苷元百分含量.PDFVIP

松木层孔菌胞外多糖的组成分析及免疫学活性研究 袁雷，钟国辉，田发益(西藏农牧学院生物技术中心，西藏 林芝 860000) 摘要：目的 研究松木层孔菌胞外多糖的单糖组成和免疫学活性。方法 使用冻融分级、酶+seveg 法脱蛋白和 Sepharose CL-6B 层析纯化松木层孔菌胞外粗多糖(CPPE) ，得到级分 PPE ，用 HPLC 分析其均一性和分子量，用气相色谱法测其单 糖组成。结果 经 HPLC 验证，PPE 为均一组分，分子量为 3.9×104 Da 。气相色谱分析表明，PPE 为甘露聚糖。淋巴细 胞增殖实验显示，PPE 能促进小鼠淋巴细胞的增殖，同时也可协同有丝分裂原 ConA 和 LPS 促进小鼠脾 T、B 淋巴细胞 的增殖。结论 PPE 具有提高机体免疫力的功能。 关键词：松木层孔菌；胞外多糖；组成分析；淋巴细胞增殖 中图分类号：R284.1 ；R285.5 文献标志码：B 文章编号：1007-7693(2010)08-0711-04 Study on Composition and Immunomodulating Activities of Extracellular Polysaccharide from Culture Medium of Phellinus pini 作者简介：袁雷，男，硕士，讲师 Tel: (0894)5831835 E-mail: yuanlei202@ 中国现代应用药学2010 年 8 月第 27 卷第 8 期 Chin JMAP, 2010 August, Vol.27 No.8 ·711 · YUAN Lei, ZHONG Guohui, TIAN Fayi(Biotechnology Center of Tibet Agricultural and Animal Husbandry College, Linzhi 860000, China) ABSTRACT: OBJECTIVE Study on monosaccharide composition and immunomodulating activities of extracellular polysaccharide of Phellinus pini . METHODS PPE was purified from CPPE using a combination of techniques, such as: frozen-thawed, deproteinated by enzyme/Sevag method and Sepharose CL-6B column chromatography. Homogenicity and molecular weight of PPE were analyzed by HPLC, and its composition was assayed by GC. RESULTS PPE appeared as single symmetrical peak on HPLC, and the average molecular weights was 39 kDa. Monosaccharide analysis by GC shows that it was mannan. The bioassay showed that PPE could stimulate mouse splenic lymphocytes proliferation and increase the ConA or LPS-induced lymphocytes proliferation in vitro. CONCLUSION The results showed that PPE have the function of enhancing the immunity. KEY WORDS: Phellinus pini; extracellular polysaccharide; composition analysis; lymphocyte transformation 松木层孔菌隶属于层孔菌属，是一种寄生于云 Vavian 3400 气相色谱仪(美国 Vaviam 公司， 杉、落叶松