正交试验优化淫羊藿总黄酮和多糖的分步提取工艺优化_付亮.pdf

56 2012, Vol.33, No.24 1 2 1 1 2 1,* (1. 625014 2. 611130) 1:10(g/mL)70%60 150W 25min4.72% 1:1090 230min1.89% Orthogonal Array Design for the Optimization of Successive Extraction of Total Flavonoids and Polysaccharides from Epimedium brevicornum Maxim 1 2 1 1 2 1,* FU Liang YUAN Jingya YANG Rui-wu ZHANG Li ZHOU Yong-hong DING Chun-bang (1. College of Life Science, Sichuan Agricultural University, Yaan 625014, China 2. Key Laboratory of Crop Germplasm Resources and Gene Improvement, Ministry of Education, Sichuan Agricultural University, Chengdu 611130, China) Abstract Objective: To optimize process conditions for the extraction of total flavonoids from the whole plant of Epimedium brevicornum Maxim and for further extraction of polysaccharides from the remaining residue. Methods: A two- step sequential extraction procedure was proposed for the extraction of total fl avonoids by ultrasonic-assisted extraction and polysaccharides by hot water extraction. Results: The optimum conditions for the extraction of total fl avonoids were found to be two extraction cycles at 60 and a microwave power of 150 W for 5 min each cycle with 70 ethanol at a solid-to- % solvent ratio of 1:10 (g/mL), resulting in an extraction effi ciency of 4.72%. The optimum conditions for the extraction of polysaccharides were found to be two extraction cycles at 90 for 30 min each cycle with a residue-to-water ratio of 1:10