烟草PR1a基因启动子的克隆及其活性检测_王云鹏.pdf

DOI :10.13207/ki.jnwafu.2011.05.002 39 　5 () Vol.39 No.5 20115 Journal of Northwest A F University(Nat.Sci.Ed.) ay 2011 ＊ PR 1a 1, 2 2 2 2 1 王云鹏 , 韦正乙, 邢少辰, 林春晶, 王丕武 (1 , 130118;2 , 130033) [ 　] 　 【】 1aPR1aP, 。【】 PCR, 1a PR1aP, (smGFP)p3300-PR1aP-GFP, , (SA)GFP , SDSELISA GFP 。 【 】, PR1aP 1 320 bp, , W-box、ACGT , as-a-likeCAAT、TATA 。 PCR , ; , PR1aP, GFP 。 SDSELISA , 72 h、0.25 mg/ L , GFP , GFP 0.083%, 15 μg/ g。 【】 1aPR1aP 。 [ ] 　; 1a ;; [ ] 　Q785 [ ] 　A [ ] 　1671-9387(2011)05-0154-07 Cloning of tobacco PR1a promoter and its act ivity evaluat ion 1, 2 2 2 WANG Yun-peng , WEI Zheng-yi , XING Shao-Chen , 2 1 LIN Chun-jing , WANG Pi-wu (1 Colleg e of Biology S ciences, J ilin A gricultural University , Chang chun, J ilin 130118, China; 2B iotechnology Research Cen tre, J ilin A cademy of Ag ricultural S ciences, Changchun, Jilin 130033, China) Abstract:【Objective】This paper aimed to clone and identify the pathogenesis-related protein 1a gene promoter (PR1aP)from tobacco.【 ethod】In this study, the promoter, PR1aP, was isolated from the ge- nomic DNA of tobacco by PCR amplification.The cloned promoter PR1aP was used to drive the soluble modified fluorescent protein (smGFP)reporter gene to construct plant expression vector p3300-PR1aP- GFP, which was introduced into tobacco via agrobacterium mediated transformation.Analysis of the smG- FP activities, induced by salicylic