音猬因子修饰纳米晶胶原基骨复合骨髓间充质干细胞修复股骨缺损.docVIP

音猬因子修饰纳米晶胶原基骨复合骨髓间充质干细胞 修复股骨缺损 唐宇星，赵 庆，杨中萌，叶永恒，胡仁安(中山大学附属第五医院骨一科，广东省珠海市 519000) 引用本文：唐宇星，赵庆，杨中萌，叶永恒，胡仁安. 音猬因子修饰纳米晶胶原基骨复合骨髓间充质干细胞修复股骨缺损[J].中国组织工程研究，2017，21():2180-2185. DOI:10.3969/j.issn.2095-4344.2017.14.008 ORCID: 0000-0001-8363-2908(唐宇星) 文章快速阅读： 文题释义： 音猬因子：作为一种Hedgehog信号蛋白，具有促使骨组织发育、生长及重构等生理功能。 纳米晶胶原基骨支架：具有优秀的生物融合性及分解性，能有效传导骨组织，并由纳米级晶体组成；其无机物分子和胶原结合构成和天然骨相似，并具有类天然三维孔洞松质骨网，且单孔大小约100 μmEffect of bone marrow mesenchymal stem cells combined with Shh modified nano-hydroxyapatite/collagen for femoral defect repair Tang Yu-xing, Zhao Qing, Yang Zhong-meng, Ye Yong-meng, Hu Ren-an (Department of Orthopedics, the Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai 519000, Guangdong Province, China) Abstract BACKGROUND: SSh as a Hedgehog signal protein can promote bone development, growth and remodeling. OBJECTIVE: To investigate the effect of bone marrow mesenchymal stem cells (BMSCs) combined with Shh modified nano-hydroxyapatite/collagen (nHAC) in the repair of femoral defects in rats. METHODS: Forty-eight Sprague-Dawley rats were randomly divided into four groups, and the model of femoral defects was established in these rats. At 14 days after modeling, experimental group was implanted with the BMSCs/Shh modified nHAC, scaffold group was implanted with simple nHAC, cell scaffold group was implanted with BMSCs/nHAC, and blank control group was without any implantation. At 3, 6, 9, 12 weeks after repair, X-ray examination, bone density measurement and bone biopsy in bone defect area were performed. RESULTS AND CONCLUSION: (1) X-ray examination: The Lane-Sandhu X-ray score and bone mineral density value in the experimental group at different time points after operation were significantly higher than those in the other three groups (P 0.05). (2) Hematoxylin-eosin staining: 12 weeks after repair, a small amount of bone tissues but no bone marrow formed in the scaffold group; a small amount of bone tissues with absence of bone marrow formed in the cell