薄荷精油提取与应用.doc

学校代码： 10722 学号： 0808014123 分 类 号: O621.4 密级： 公开 薄荷精油的提取及其杀菌活性的研究 Extraction of Essential oils from Peppermint And its Bactericidal Activity 作者姓名 张 斌 作者姓名 专业名称化 学 专业名称 学科门类 理 学 学科门类 提交论文日期指导教师 张知侠 提交论文日期 指导教师 2012年5月 成绩评定 成绩评定 摘 要 植物精油是植物体内小分子化合物形成的混合物，不同的成分组成有不同的功效。由于传统的化学农药有严重的残留问题，对人体和环境伤害较大；因此，近年来有许多人对精油的杀菌活性作了研究，用精油代替农药以减小对人体和环境的伤害。本实验采用水蒸气蒸馏法提取薄荷中的精油，再用GC-MS检测其化学组成成分，最后用速率测试法来研究薄荷精油的杀菌活性。薄荷精油中薄荷醇含量最高为44.34%，邻苯二甲酸二丁酯为7.84%，1,3-二氧六环-5-醇为7.59%，桉树醇为7.24%，5-甲基-2-异丙基环己酮为5.51%，α-萜品醇为4.92%，三甲基苯甲醇为4.47%。用薄荷精油对十二种真菌做杀菌活性实验，其对不同真菌的杀菌活性有所不同，其中在2500 mg/L下对水稻纹枯，玉米大斑，黄瓜炭疽，苹果炭疽，小麦赤霉，番茄叶霉的杀菌活性都在93.02%以上。在1500mg/L对番茄早疫和在500mg/L对小麦纹枯的杀菌活性都为100%。 关键词：薄荷；植物精油；水蒸气蒸馏；GC-MS；杀菌活性 Abstract Plant essential oil in plants is small molecules form, a mixture of different compositions have different effect. Because the traditional chemical pesticide residues have serious problems, the damage to the body and the environment is bigger; Therefore, in recent years, many of the essential oil to the bactericidal activity study, with the essential oil instead of pesticides to minimize the damage to the body and the environment. The experimental results of the essential oil extraction steam distillation menthol, again by GC-MS testing its chemical composition, final with speed rate testing method to study the peppermint oil bactericidal activity.Menthomenthol is the highest content as 44.34% in peppermint oil, dibutyl phthalate as 7.84%, 1,3-Dioxan-5-ol as 7.59%, 1,8-Oxido-p-menthane as 7.24%, p-Menthan-3-one as 5.51%, p-menth-1-en-8-ol as 4.92%, p-Cymen-3-ol as 4.47%. Use of peppermint oil to twelve kinds of fungi do bactericidal activity experiment, it to the different fungi bactericidal activity is different, which in 2500 mg/L bactericidal activity of peppermint oil to Setosphaeria turcica,Rhizocto