邻苯二甲酸乙基已基酯dehp对小白鼠肝脏毒性及-生态毒理学报.doc

邻苯二甲酸(2-乙基已基)酯(DEHP)对小白鼠肝脏毒性及脂质过氧化损伤 陈文婕1　戴红　陈敏2　吴茂聪3　刘鹏3　胡月玲1 内蒙古医学院公共卫生学院环境卫生(职业医学教研室，内蒙古呼和浩特 (010059) 大连医科大学公共卫生学院食品与营养学教研室，辽宁大连 (116044) 大连医科大学公共卫生学院劳动卫生(环境卫生教研室，辽宁大连 (116044) 摘要：为了研究邻苯二甲酸(2-乙基已基)酯（DEHP）对小鼠肝脏的毒性及脂质过氧化损伤作用机制，选择昆明4周龄小鼠80只，雌雄各半；随机分为4组。经食饵连续自然给食染毒，于染毒第4周末处死。测量小鼠肝脏和体重的变化，测定不同DEHP染毒剂量组小鼠的血液、肝脏中谷胱甘肽过氧化物酶（GPX），过氧化氢（H2O2）,丙二醛（MDA）,超氧化物歧化酶(SOD)等变化。将实验数据进行ANOVA分析处理后，结果表明，DEHPP0.01)。苏木精-伊红染色法（简称HE染色）可见高剂量组肝脏组织有明显损伤。与对照组相比，DEHP三个剂量染毒组小鼠血液（A组除外）及肝脏中GPX活性降低（P0.05，P0.01），H2O2含量增加（P0.05，P0.01）；肝组织中(A组除外)SOD活性降低（P0.05，P0.01），MDA含量增加(P0.01）。结果表明DEHP对小鼠肝脏的毒性作用机制可能为脂质过氧化反应。 关键词：DEHP；肝脏 ；脂质过氧化损伤 Hepatotoxicity and Lipid peraxidation of Mice Induced by Diethylhexyl phthalate Chen wenjie1 Dai Hong1 Chen Min2 Wu maocong3 Liu Peng3 Hu yuelin1 Department of Occupational and Environmental Health, School of Public Health, Inner Mongolia Medical College, Hohhot, Inner Mongolia(010059) Department of Food and Nutrition, School of Public Health,Dalian Medical University,Dalian,LiaoNing(116044) Department of Occupational and Environmental Health, School of Public Health, Dalian Medical University,Dalian,LiaoNing(116044) Abstract: To study the liver toxicity mechanism and lipid peroxidation induced by diethylhexyl phthalate(DEHP) on mice,Eighty of Kumming mice were divided into 4 groups randomly, male and female half and half.The animals were exposed to DEHP by food for 4 weeks.And the end of the fourth week,they were sacrificed.Then detected the change of liver and weight on mice,in the meantime,the activities of GPX and SOD in the blood and liver tissue were measured,the content of H2O2 and MDA in the blood and liver tissue were also measured. The results of ANOVA test show that the weight of mice decreased significantly with increase of DEHP(P0.01),and the coefficient of liver/body with high dose group increased significantly.There were evident liver tissue damage in high dose group by HE staining.The GPX activities of DEHP exposure groups in the blood tissue(except the g