解淀粉芽胞杆菌pc2产抑菌物质培养基及发酵条件优化.pdfVIP

微生物学通报 Jun. 20, 2017, 44(6): 1358−1369 Microbiology China /wswxtbcn tongbao@ DOI: 10.13344/j.microbiol.china.160607 研究报告 解淀粉芽胞杆菌 PC2 产抑菌物质培养基及发酵条件优化 * 申光辉 郑丽君 张志清 程璐 杨红霞 吴贺君 黎杉珊 ( 625014) 摘 要：【目的】优化解淀粉芽胞杆菌PC2 产抑菌活性物质发酵培养基及发酵条件。【方法】以 马铃薯葡萄糖液体培养基为基础，依据发酵液对金黄色葡萄球菌抑菌圈的单因素试验结果，采用 Box-Behnken 响应面法优化发酵培养基，二次通用旋转组合设计，频率分析法优化发酵条件。【结 果】影响发酵液抑菌活性的培养基主要组分为马铃薯、蔗糖和 L-谷氨酸钠，最优发酵培养基配 方为：马铃薯188.0 g/L，蔗糖22.0 g/L ，L-谷氨酸钠 1.80 g/L，培养基成本为0.81 元/L ；最佳发 酵条件为：接种量6%、发酵温度30 °C、装液量40 mL/250 mL 、摇床转速185 r/min、发酵时间 24 h 、初始pH 7.0 。优化后发酵液对金黄色葡萄球菌抑菌圈直径为30.82 mm ，较优化前的18.22 mm 增加了 12.60 mm。【结论】优化后的培养基和发酵条件提高了解淀粉芽胞杆菌PC2 发酵液的抑菌 活性，为该菌株的工业化生产应用提供了依据。 关键词：解淀粉芽胞杆菌，抑菌物质，发酵条件，响应面法，二次通用旋转组合设计，优化 Optimization of fermentation medium and conditions for antimicrobial substances production by Bacillus amyloliquefaciens PC2 * SHEN Guang-Hui ZHENG Li-Jun ZHANG Zhi-Qing CHENG Lu YANG Hong-Xia WU He-Jun LI Shan-Shan (College of Food Science, Sichuan Agricultural University, Ya’an, Sichuan 625014, China) Abstract: [Objective] To improve antimicrobial activity of Bacillus amyloliquefaciens strain PC2, the medium composition and fermentation conditions were optimized in our work. [Methods] Single factor experiments were carried to evaluate the effect of alternative constitutes for PDB medium on inhibition zone diameter against Staphylococcus aureus, Box-Behnken response surface methodology was conducted to obtain the optimal composition of the crucial medium factors. The optimal fermentation c