Bradford Reagent (B6916) Technical Bulletin （布拉德福德试剂(B6916)技术通报）.pdf

Bradford Reagent Catalog Number B6916 Storage Temperature 2–8 C TECHNICAL BULLETIN Product Description Protein Standard (BSA) Solution, (2 mg/ml), The Bradford Reagent can be used to determine the Catalog Number P0834 concentration of proteins in solution. The procedure is Protein Standard (BSA) Solution, (1 mg/ml), based on the formation of a complex between the dye, Catalog Number P0914, if lower concentrations of Brilliant Blue G, and proteins in solution. The protein- protein are to be measured. dye complex causes a shift in the absorption maximum of the dye from 465 to 595 nm. The amount of Precautions and Disclaimer 1 absorption is proportional to the protein present. The This product is for RD use only, not for drug, Bradford Reagent requires no dilution and is suitable for household, or other uses. Please consult the Material micro, multiwell plate, and standard assays. The linear Safety Data Sheet for information regarding hazards concentration range is 0.1– 1.4 mg/ml of protein, using and safe handling practices. BSA (bovine serum albumin) as the standard protein. Storage/Stability The Bradford Reagent is compatible with reducing The product is stored at 2–8 C. It is stable at 2–8 C in agents. Reducing agents are often used to stabilize an unopened container for at least 1 year. proteins in solution. Other protein assay procedures (Lowry and BCA) are not compatible with reducing Procedure agents. The Bradford Reagent should be used in place The standard 3.1 ml Bradford assay consi