Computergenerated threedimensional （电脑生成的三维）.pdf

Computer-generated three-dimensional representation of dictyosomes reconstructed from electron micrographs of serial ultrathin sections U. KRISTEN*, J. LOCKHAUSEN Institut filr Allgemeine Botanik, Universitdt Hamburg, Ohnhorststrasse 18, D-2000 Hamburg 52, West Germany W.MENHARDT Philips GmbH, Fonchungslabor Hamburg, Postfach 540840, D-2000 Hamburg 54, West Germany and W. J. DALLAS Department of Radiology, University of Arizona, Health Sciences Center, Tucson, Arizona 85724, USA * Author for correspondence Summary Computer-generated three-dimensional reconstruc- tubular protrusions. The entire dictyosome is tions of higher plant dictyosomes from electron suggested as being an approximately spherical micrographs are presented from different viewing body that is marginally penetrated by plates and angles in the form of shaded perspective displays. tubules of the endoplasmic reticulum. The image processing has revealed complicated structures indicating a central cisternal stack sur- Key words : Golgi apparatus , three-dimensional rounded by a cloud of vesicles and vesicular and reconstruction, computer image display . Introduction shaded perspective display of more complex structures, e.g. the network of endoplasmic reticulum or the stacks of The classical methods for converting electron micro- Golgi cisternae forming a dictyosome, a high degree of graphs into stereographic representation of cell com- interactivity has to be integrated into the im