耐盐紫甘薯花色苷的纯化工艺研究.PDFVIP

耐盐紫甘薯花色苷的纯化工艺研究  孙梦茹，朱大伟，王明丽(常州市第一人民医院，江苏 常州 213003) 摘要：目的 建立有效的紫甘薯花色苷提取物分离纯化方法，为其产业化发展提供理论依据。方法 通过静态吸附解吸 试验筛选适合耐盐紫甘薯 Z103 花色苷纯化的大孔树脂，并进行动态吸附解吸工艺条件优化。结果 在供试树脂中， HPD-300 大孔树脂纯化效果最好。其静态吸附达平衡的时间为6~8 h ，解吸附平衡时间为1~2 h，最大吸附量为33.67 mg·g1。 1 1 动态吸附解析最适工艺条件为：上样流速 1.0 mL·min ，上样浓度为0.3 mg·mL ，体积为145 mL 时，HPD-300 树脂动 1 1 态最大吸附量为13.82 mg·g (树脂) ，采用80%乙醇溶液作为洗脱剂，洗脱流速1.0 mL·min ，用3 倍柱体积洗脱花色苷， 1 ，比纯化前提高13.18 倍；色价 1% (528 nm)为142.63， 收率可达92.01% 。纯化后的紫甘薯花色苷含量为181.58 mg·g E 1 cm 比纯化前提高11.01 倍。结论 该工艺具有较好的实用性与参考价值。 关键词：紫甘薯；花色苷；纯化；大孔树脂 中图分类号：TQ460.6 文献标识码：A 文章编号：1007-7693(2013)12-1323-08 Study on the Purification Craft of Anthocyanins from Salt-tolerant Purple Sweet Potato SUN Mengru, ZHU Dawei, WANG Mingli(The First People’s Hospital of Changzhou, Changzhou 213003, China) ABSTRACT: OBJECTIVE To establish an efficient method for isolating and purifying anthocyanins from purple sweet potato, provide a theoretical basis for its industrial development. METHODS The static adsorption and desorption tests had been done to screen for the optimum macroporous resin and condition for purifying anthocyanins extracted from salt-tolerant purple sweet potato Z103, the dynamic adsorption and desorption properties of the resins were studied additionally. RESULTS The macroporous resin HPD-300 was the most favorable adsorbent. The time of absorption and desorption equilibrium was 68 h and 12 h, respectively. The absorpti