妊娠后期宫内生长受限对蒙古绵羊胎儿肌肉和肾脏脂肪组织生长发育影响.pdfVIP

本论文由国家自然科学基金项目和内 蒙古自然科学基金项目（2009BS0405）资助，特此感谢。 摘 要 本实验旨在研究妊娠后期IUGR 对蒙古绵羊胎儿肌肉及肾脏脂肪组织生长发育 的影响。选择体况中等、2~3 胎次，经同期发情且受孕，平均体重为53.28±4.87kg， 健康的蒙古绵羊42 只。在妊娠90 d 时选择6 只具有代表性的母羊进行屠宰，其余 -0.75 -1 -0.75 -1 按体重随机分配到三个组：0.175 MJ·kgw ·d （RG1 ：n=14 ）、0.33 MJ·kgw ·d -0.75 -1 （RG2 ：n=12 ）和自由采食组(0.67 MJ·kgw ·d ，CG ：n=10 ），进行不同能量水平 饲养至妊娠140 d，各组再选择6 只具有代表性的母羊进行屠宰，取出胎儿进行屠宰 并获得背最长肌、半腱肌、股二头肌、臂三头肌和肾脂。结果表明： （1）妊娠后期随着母体营养水平的降低，限制组胎儿的体重、平均日增重显著 降低。 （2）随着母体营养水平的降低，RG2、RG1 组胎儿酮体、酮体组分、背最长肌、 半腱肌、股二头肌、臂三头肌重显著降低；背最长肌、臂三头肌肌纤维的密度显著 升高，而面积、直径显著降低；半腱肌、股二头肌肌纤维的密度显著降低。 （3）随着母体营养水平的降低，CG、RG2、RG1 组胎儿肾脂重、肾脂线粒体总 DNA 含量、 UCP1 基因的表达量显著下降；肾脂细胞中线粒体蛋白浓度、Leptin 基 因的表达量上升，肾脂结构改变。 关键词：宫内生长受限；绵羊胎儿；肌肉；肾脂 Effects of Intrauterine Growth Restriction during Late Pregnancy on the Growth and Development of Ovine Fetal Muscle and Perirenal Adipose Tissue Abstract This study investigated the effects of intrauterine growth restriction during late pregnancy on the growth and development of ovine fetal muscle and perirenal adipose tissue. 42 Mongolian ewes in their second or third parity, which had similar live weight (mean 53.28±4.87kg) were mated at a synchronized oestrus. 6 ewes were slaughtered at the beginning of the experiment and the remaining 36 animals were allocated to three different groups: Restricted Group1 (RG1, 0.175 MJME kgw-0.75d-1, n=14), Restricted Group2 (RG2, 0.33 MJME kgw-0.75d-1, n=12) and Control Group (CG, ad libitum, 0.67 MJ ME kgw-0.75d-1, n=10). At 140d of gestation, 6 representative ewes in each group were slaughtered, and their fetal longissimus dorsi muscle, s