Bio-plex悬浮芯片技术检测两种试验兔白介素基因-中国试验动物学报.DOC

Bio-Plex悬浮芯片检测两种实验兔白介素基因( （浙江中医药大学动物实验研究中心/比较医学研究中心，杭州 310053） 【摘要】目的 采用液相悬浮芯片同时测定兔圆小囊中IL1β、IL1R1、IL8、IL8RA和IL15各基因的表达情况，并对该方法进行评价。方法 利用Affymetrix的Panomics QuantiGene Plex 2.0 Assay中bDNA信号放大和多磁珠分析技术，来同时检测多重mRNA并定量。结果 可同时检测IL1β、IL1R1、IL8、IL8R和IL15各基因的含量发现兔IL15基因的相对表达量显著高于兔（P0.05），IL1R1基因的相对表达量显著高于兔（P0.01）IL-8RA基因兔的相对表达量高于兔（P0.05）。结论 IL-15、IL-1R1和IL-8RA基因WHBE兔免疫学特性 【关键词】 液相悬浮芯片；定量；多重基因表达检测；bDNA；磁珠阵列 of Two Rabbits Interleukin Gene by the Bio-Plex Suspension Array System MAN Man, CAI Yue-qin, XU Jian-qin, CHEN Cheng, HUANG Yu, ZHAO Hong-shu, YANG Qin-qin, CHEN Min-li （Laboratory Animal Research Center, Zhejiang Chinese Medical University , Hangzhou 310053, China） 【Abstract】 Objective To measure IL1β, IL-1R1, IL-8, IL-8R and IL-15 genes expression in rabbit sacculus rotundus using the Liquid suspension array system, and evaluate the method. Methods The simultaneous measurement and quantity of multiple genes mRNA expression in rabbit sacculus rotundus was detected by the Panomics QuantiGene Plex 2.0 Assay of Affymetrix using the bDNA signal amplification and beads analysis techniques. Then, this method was applied in the detection of rabbit interleukin gene. Results Content of IL-1β, IL-1R1, IL-8, IL-8RA and IL-15 gene can be detected simultaneously. Gene expression of IL-15 in WHBE rabbit is significantly higher than JW rabbit (P0.05), and gene expression of IL-1R1 in WHBE rabbit is significantly higher than JW rabbit (P0.01). Also, the relative expression quantity of IL-8RA gene in WHBE rabbit is higher than JW rabbit (P0.05). Conclusion Liquid suspension array method for detecting rabbit interleukin gene was established. Gene expressions of IL-15, IL-1R1 and IL-8RA in WHBE rabbit were higher, and all the results reveal WHBE rabbit may have unique immunological properties. 【Key words】Liquid suspension arrayuantitative；Multi-gene expression detection；bDNA；Bead array 白毛黑眼兔（White hair black eyes rabbit，简称WHBE兔）是敏感、制备抗血清效价高而被免疫学和分子生物学研究者所青睐， [1-3]。虽然其遗传性状、血液学和生理学方面的研究有所报道