蛋白质芯片揭示PN-1的多靶位治疗作用-中国试验动物学报.DOC

中药I号方对阿尔兹海默病的多靶位治疗作用 梁良，刘羽，杨亚军，朱华，黄澜，盛树力，秦川，邓巍 （北京协和医学院 中国医学科学院医学实验动物研究所病理室，卫生部人类疾病比较医学重点实验室， 国家中医药管理局人类疾病动物模型三级实验室北京 100021） 【摘要】 目的 应用高通量抗体芯片技术（KinexTM Antibody Microarray）来研究中药I号方（PN-1）对阿尔兹海默病的治疗作用。 方法 将阿尔兹海默病模型小鼠分为两组，其中对照组4只，每天给予双蒸水灌胃，给药组8只每天给予中药提取物灌胃，给药3个月后，利用KinexTM Antibody Microarray 筛选出两组的差异表达蛋白，然后用Ingenuity Pathway Analysis（IPA）及查阅文献找到其中与阿尔兹海默病相关的蛋白分子。 结果 给予中药I号方（PN-1）的阿尔兹海默病小鼠与空白对照小鼠比较，差异表达蛋白其中与阿尔兹海默病相关，且存在变化的为：p- tau（T522）、COX2、 caspase6和caspase9下降；bcl-xl 和CaMK4升高。结论 中药I号方（PN-1）能从抗tau蛋白高磷酸化、抗炎、抑制凋亡和提高记忆力等多靶位改善阿尔兹海默病模型小鼠的病理生理表现，可能为治疗阿尔兹海默病的有效药物。 【关键词】阿尔兹海默病模型小鼠；中药I号方；KinexTM Antibody Microarray；多靶位 The multitargeted therapeutical effects of Prescription No. 1 (PN-1) on Alzheimer’s disease Liang Liang1,Liu Yu 1, Cao Zi-qing2,Yang Ya-jun 1,Zhu Hua 1,Huang Lan1, Sheng Shu-li1, Qin Chuan1, Deng Wei1 (1.Comparative Medicine Center, Peking Union Medical College (PUMC);Institute of Laboratory Animal Science, Chinese Academy of Medical Science (CAMS);Key Laboratory of Human Disease Comparative Medicine,Ministry of Health;Key Laboratory of Human Disease Animal Model,State Administration of Traditional Chinese Medicine,Beijing 100021, China；2.Herbal Medicine Institute in Xijie Quwo,Shanxi 043400) 【Abstract】Objective To investigated the therapeutical effects of Prescription No. 1 (PN-1) on Alzheimer’s disease by KinexTM Antibody Microarray which is a kind of high-throughput antybody microarray. Methods The Alzheimer’s disease mouse models are divided into treatment group and control group. There are 8 mouse in the treatment group and given once PN-1 every day by intragastric administration for 3 months，The 4 in the control group are given double distilled water every day by intragastric administration for 3 months too.The different expression protein between the two groups are screened by KinexTM Antibody Microarray , the molecules relevant Alzheimer’s disease is determined by Ingenuity Pathway Analysis（IPA）and critical review of liter