雄性不育大鼠近交系MIJ的建立及其遗传特征观察.PDFVIP

Chinese Journal of Zoology 2008, 43( 5) : 37 ~ 44 MIJ * 刘军须 蔡月花 张敬各 刘福英 ( 0500 17; 0500 17) : , , MIJ , , , MIJ ; ; , : ; ; ; ; : Q321+ . 7 , Q38 : A : 2008) 05370 8 Establishment and Genetic Characteristics of Rat Inbred Strain MIJ with Spontaneous Male Infertility * LIU JunXu CAI YueHua ZHANG JingGe LIU FuYing ( Department of Laborat ory A nimal Sci ence, H ebei Medical University , Hebei Key Lab of Laboratory A nimal Science , Shi iaz huang 050017; Faculty of Forensic Medici ne ,H ebei Medical Universi ty , Shi iaz huang 050017, China) Abstract: The normal sibling rats o the in ertile male were selected or brothersister breeding. The stocks o every ilial generation whose male siblings w ere in ertile w ere inbred w ith their brothers or sisters by optimum seeking method. A ter 20 generations, a rat inbred strain o spontaneous male in ertility w as established , and w as named MIJ. The genetic homozygosity w as analyzed by skin gra ting . The phenotypes o MIJ were observed, and the mode o in ertility inheritance w as studied by test cross experiments. The results demonstrated that MIJ rats had reached the norm o inbred strain, and the in ertile male rats show ed the phenotypes o delayed testicular descent , testicular and epididymis hypoevolutism, oligozoospermia and hypospermatogenesis. The phenotypes o the other rats in the inbred strain w ere normal. The in ertility mutation w as inherited in a recessive mode, and was controlled by a