.单克隆抗体与多克隆抗体配对ELISA方法比较.pdfVIP

生物技术通报 # # BIO TE CHN OLOG Y B ULLETIN 2009 11 ELISA 1 2 1 1 张小兵 邸禄芹 吴萌 闫静辉 1 2 ( , 050081; , 050051) : 以人绒毛膜促性腺激素( HCG)为抗原, 制备出对HCG的多克 抗体和特异性单克 抗体, 并进行抗体纯化和 特性分析, 利用辣根过氧化物酶( HRP)分别对其进行了标记采用双抗夹心 ELISA试验, 探讨了多克 抗体与单克 抗体配 对的若干事项结果表明, 利用单克 抗体和酶标多克 抗体配对, 并用含动物血清的稀释液稀释酶标抗体, 可实现对检测 原的高特异性和高灵敏度检测 : 单克 抗体 多克 抗体 抗体配对 ELISA 法 Study of the Optimum Pairing of PolyclonalAntibodies and MonoclonalAntibodies with Sandwich ELISA 1 2 1 1 Zhang X iaobing D iL q in W M eng Yan Jingh i 1 ( B iology Institute of H ebei , Sh ij iazhuang 050081; 2 The Th irdH osp ital of H ebeiM edical University, Sh ij iazhuang 050051 Abstrac:t Us ing h m an chorionic gonadotroph in( HCG) as the antigen, polyclonal antibod ies and monoclonal antibodies( M cAbs) specific forH CG were generated, characterized and high ly p rified. Then these antibod ies were labeled w ith horserad ish peroxidase ( HRP). By a do ble antibody sandw ich enzym e-linked mi m nosorhent assay, several problem s of the optmi m pairing of polyclonal an- tibod ies and m onoclonal antibod ies w ere disc ssed. The res lts showed that w ith m onoclonal antibody as trapp ing antibody and poly- clonal antibody labeling w ith enzym e as testing an tibody wh ich sho ld be dil tedw ith an mi al sera as d il ent, the sandw ich EL ISA had a h ighly specificity and sensitivity to detect antigen. K ey w ords: M onoclonal antibody Polyclonal antibody Optmi m pa iring of antibodies Sandw ich EL ISA ,