大豆分枝数和叶柄夹角的相关野生片段分析王吴彬.pdfVIP

2012,45(23):4749-4758 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2012.23.001 /// 210095 Detection of Wild Segments Associated with Number of Branches on Main Stem and Leafstalk Angle in Soybean WANG Wu-bin, HE Qing-yuan, YANG Hong-yan, XIANG Shi-hua, ZHAO Tuan-jie, XING Guang-nan, GAI Jun-yi (Soybean Research Institute, Nanjing Agricultural University/National Center for Soybean Improvement/MOA Key Laboratory for Biology and Genetic Improvement of Soybean (General)/National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095) Abstract: Objective The number of branches on main stem (NB) and leafstalk angle (LA) are important factors for plant type and therefore, closely related to yield potential in soybean. The wild soybean (Glycine soja Sieb et Zucc.) usually has the characteristics of multiple NB and wide LA, of which the genetic construction is of relevant meaning in the improvement of yield potential of soybean. The present study was aimed at detecting the wild segments related to the two traits and evaluating their genetic effects from a wild soybean chromosome segment substitution line population (CSSL) with cultivated soybean genetic background, so as to provide genetic materials for further gene cloning and its function study. Method A wild soybean CSSL population with 151 lines, designated as SojaCSSLP1 was used to identify the wild alleles/segments for NB and LA by using joint comparisons of CSSLs significantly different from the recurrent parent based on QTL mapping with the methods of single marker analysis (SMA), interval mapping (IM), inclusive composite interval mapping (ICIM) and mixed linear composite interval ma