18 184-187 接168 工艺技术 4-16 棘孢小单孢菌原生质体制备条件的优化.pdf

现代食品科技 Modern Food Science and Technology 2010, Vol.26, No.2 棘孢小单孢菌原生质体制备条件的优化 高辉，敖康，涂国全 （江西农业大学生物科学与工程学院，江西南昌330045） 摘要：对棘孢小单孢菌(Micromonospora echinospora)原生质体制备条件进行了研究，研究表明：在种子培养基中加入0.3 ％甘氨酸最有利于原生质体的制备，收集培养7 2 h 的棘孢小单孢菌菌丝体，在37 ℃下用20 mg/ml溶菌酶酶解90 min ，此时原生质体形成率与再生率之积最高，效果最好。 关键词：棘孢小单孢菌；原生质体；制备；优化 文章篇号:1673-9078(2010)2-184-187 Optimization of Preparation of Protoplast from Micromonospara echinospora GAO Hui, AO Kang, TU Guo-quan (Department of Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China) Abstract: The preparation conditions of protoplasts of Micromonospara echinospora were studied and optimized. Research showed that adding 4 % glycine to culture medium was the most suitable for protoplasts preparation from Micromonospara echinospora. The highest product of the generation rate and regeneration rate was achieved by collecting mycelium from Micromonospara echinospora cultured for 72 hours and enzymatically hydrolyzing by 20 mg/ml of iysozyme at 37 ℃for 90 min. Key words: Micromonospora echinospora; protoplasts; preparation; optimization 184 通讯作者：涂国全教授 棘孢小单孢菌（Micromonospara 4 的主要因素，并采用L (3 )正交试验对原生质 9 echinospora）既产小诺霉素(Micronomicin , 体的制备条件进行优化。 MCR) ，又产庆大霉素(Gentamicin, GM)，其产物小诺霉素（图1）又称相模湾霉素( Sagamicin, SGM)，即庆大霉素（图2）C2b组分(即庆大霉素 C1a组分的6’- 图1 小诺霉素 图2 庆大霉素 Fig.1 Structure of Micronomicin Fig.2 Structur