基因工程考试名词解释（Genetic engineering examination noun explanation）.docVIP

基因工程考试名词解释（Genetic engineering examination noun explanation） 3, Alpha complementary (Alpha complementation) : phage carrier gene spacer into the e.c. with our fabrication: oli a regulatory gene and the N end of lac Z gene encoding 146 amino acid residues, its encoding product for Alpha beta - galactose glucoside enzyme fragment. The mutant lac-e.c oli can express the W fragment of the enzyme (the C end of the enzyme). Separate segments of alpha and W no beta galactose glucoside enzyme activity, only the host cell and the cloning vector were two pieces at the same time, the host cells have a beta galactose glucoside enzyme activity, make specific agents, a blue compounds, this is known as alpha complement each other. Alkaline phosphatase (Alkaline phosphatase) : an enzyme that removes the phosphate root of DNA/RNA 5 . When the carrier is prepared, it can be treated with alkaline phosphatase to prevent its own connection and improve the efficiency of recombination. 10. Autoradiography: techniques for the observation and quantitative detection of molecules with radiological activity by exposure to the X-ray membrane. Phage (Phage) : a virus that infects bacteria. 12. Blot (Blot) : the technology of biological macromolecules that are present in gelation in the immobilized media and tested and analyzed. 13, Blue White filter (Blue/White Cloning) : when the host bacterium infection by a phage, both by alpha complementary effect, can produce a beta galactose glucoside enzyme activity, the inducer IPTG and artificial substrate X - gal to exist, to produce a Blue plaque. If an exogenous DNA fragment is inserted into the phage vector, the structure of the lac gene can be destroyed, and it cannot be complemented with the beta-galactosidase in host bacteria, and the white colony is produced when IPTG and x-gal exist. Because of the color mark, the separation between recombinant clones and non-recombinant clones is clear, and the screening method is called a blue and white spot