色谱常识（Chromatography common sense）.docVIP

色谱常识（Chromatography common sense） Reasons for tailing of chromatography and Solutions 1, when the maintenance chromatography problem (peak tailing, sensitivity decrease, retention time change etc.), Cut to the front of the column of the 1/2 to 1 meters. If necessary, replace the liner and spacer in the inlet, And clean the sample inlet. The protection column can be used to increase the service life of the chromatographic column. (gas phase) 2 、 liner deactivation The active point on the injection liner can adsorb sample components and cause trailing peaks, and may lose sensitivity and reproducibility. An active preparation is used to cover or react with the active point on the lining glass surface. The deactivation process enables the liner to be reproducible, inert and has a long service life. For non shunt applications, or if a compound with a weak polarity must be analyzed, a deactivated liner shall be used. Even if the liner is removed, it will begin to show activity, and when this happens, the liner shall be replaced. The liner can be cleaned to remove particulates or to flush the liner with solvent to remove the less volatile components. However, it may be difficult to select the appropriate lining cleaning procedure. Certain solvents may remove the deactivation layer, and the tool may scratch the glass surface of the liner, resulting in unwanted active spots. The new liner is almost always better than the cleaned and re - activated liner - especially for trace analysis. 3. Chromatographic columns, inlet liners, or contaminated metal inlet seals. Samples of absorbed components are lined with new liners or cleaned with old tubes and replaced with glass wool. The injection needle strikes the inside of the inlet liner Crushing of filling. Remove part of the filling from the liner or use a non filling liner. The end of the chromatographic column is not neatly cut (samples are adsorbed here) and the chromatographic column is removed. Using a secure capillary fused