BG11液体培养配方的.doc

BG11液体培养基就是： Stock1 定容100mL 柠檬酸 0.3g 柠檬酸铁胺 0.3g EDTANa2 0.05g Stock2 定容1000mL NaNO3 30g K2HPO4 0.78g MgSO4·7H2O 1.5g Stock3 定容100mL CaCl2·2H2O 1.9g Stock4 定容100mL Na2CO3 2g Stock5 定容1000mL H3BO3 2.86g MnCl2·4H2O 1.81g ZnSO4·7H2O 0.222g Na2MnO4·2H2O 0.391g CuSO4·5H2O 0.079g Co(NO3)2·6H2O 0.049g Stock1 取用2mL Stock2 取用20mL Stock3 取用2mL Stock4 取用1mL Stock5 取用1mL 总定容 1000mL D1 (Diatom medium) ? Component ????????????Amount ????????Stock Solution???? (1) NaNO3??? ???????????????1 mL/L????????????12 g/100ml dH2O ????????? (2) K2HPO4 ?????????????????1 mL/L ????????????4 g/100 mL dH2O??? ????? (3) MgSO4·7H2O?????????1 mL/L??????????? ?7g/100 mL dH2O ???????? (4 ) CaCl2·2H2O??????????1 mL/L ????????????2 g/100 mL dH2O?????? (5 ) KH2PO4???????????????? 1 mL/L ????????????8 g/100 mL dH2O ??????? (6 ) Na2SiO3.9H2O????????1 mL/L???? ??????? 10g/100ml dH2O (7) MnSO4????? ?????????? ??0.1mL/L????????????0.2g/100ml dH2O (8) 柠檬酸铁???????????????? 1 mL/L????????????? 0.5g/100ml dH2O (9) A5 （Trace mental solution）? 1ml/L???????? ????????? H3BO3??? ??????????????????2.86g/L dH2O ????????? MnCl2·4H2O??????????????1.86g/L dH2O ????????? ZnSO4·7H2O????????????? 0.22g/L dH2O ????????? Na2MoO4.2H2O????????????0.39g/L dH2O ????????? CuSO4. 5 H2O??????????????0.08g/L dH2O ????????? Co(NO3)2.6 H2O???????????0.05g/L dH2O (10) 土壤提取液?????????????? 20 mL/L ? 土壤提取液配制方法: 取花园土未施过肥200g置于烧杯或三角瓶中，加入蒸馏水1000毫升，瓶口用透气塞封口，在水浴中沸水加热3小时，冷却，沉淀24小时，此过程连续进行 3次，然后过滤，取上清液，于高压灭菌锅中灭菌后于4℃冰箱中保存备用。 ?f/2? medium Component????????????????????????? ? Amount???????? ?? (1)? NaNO3 ?????????????????????? 75 mg/L (2)? NaH2PO4 · 2H2O???????? ?0.6 mg/L (3)? Vitamin B12 ??????????????? ?0.5 μg/L (4)?? Biotin ?????????????????????? ?0.5 μg/L (5)? Thiamine HCl ????????????? ?100μg/L (6) ?Na2SiO3 · 9H2O??????????10 mg/L (7)?? f/2 metals ?????????????????1 mL/L ??????? Na2EDTA · 2H2O ?????4.4g ??????? FeCl3 · 6H2O ?????? ? ?3.16 g ??????? CoSO4 · 7H2O ??????? 0.012g ??????? ZnSO4 · 7H2O?????? ? 0.0