大肠杆菌培养基的优的化及发酵罐操作.pptVIP

大肠杆菌培养基的优化及摇瓶种子制备;目录;培养基的优化;LB培养基配方;;优化接种量;操作过程及注意事项;Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.;将配置好的种子瓶放置在摇床上培养12至24小时，设置摇床转速为180r/min，温度为37摄氏度 注意：在放置时，不要将摇瓶卡的太紧，防止摇瓶在摇床运转过程中破裂。;操作中得失误;;LB培养基的制备;40ml制种子;高压灭菌;分离单菌落;四区划线;将制作好的平板放入恒温箱中培养24至48小时，温度控制在37摄氏度。;观察结果;Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.;种子制备;操作中得失误;生长曲线;Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.;Evaluation only