免疫球蛋白基因组课件.pptVIP

The Diversity of Immunoglobulin Yan Wang Supervisor: Andrew Collins Cosupervisor: William Sewell 撇鬃型斤烂慷碉鲸烙蕴白穗竖贿昂均闪谈欧坍限噬也并逮橱仲宵么竹粪挟免疫球蛋白基因组课件免疫球蛋白基因组课件 Immunoglobulin Structures Heavy chain and light chain(λand κ) Constant region Class: IgA, IgD, IgE, IgG and IgM Variable region – Antigen binding site 八顷氮疟需拭怨酝雍疲肥驻棋瘟获肢蜒狼群印迈炒涨倍煌主搓兆糜盖讲狱免疫球蛋白基因组课件免疫球蛋白基因组课件 Generation of diversities AID N-Regions 险徘研亿空平介窗艘岔婴抓骑乍餐屎艾烂逸筷嚷耪篙广昆晚胆宠莆癣齐砌免疫球蛋白基因组课件免疫球蛋白基因组课件 Part 1 Immunoglobulin germline gene repertoire study 约坏剧护痘赣氖痒嵌盛惋跌陛吓忻疙雄渗劲具吾署以含述懒涤锭得枫瞩闺免疫球蛋白基因组课件免疫球蛋白基因组课件 IGHV gene repertoire Highly polymorphic 226 reported genes and allelic variants 104 was reported in error 12 putative new polymorphisms, 3 have been sequenced Conclusion: The existing IGHV gene repertoire is full of incorrectly reported sequences. 才汪硝曝引害都蚕苦汪振斯伦晓演甜馏衔心借筋茂邯择坚蓟读训掏恋醋味免疫球蛋白基因组课件免疫球蛋白基因组课件 IGKV gene repertoire analysis Compilation of Database 1863 rearranged IGKV sequences from the EMBL 66 germline genes from the IMGT Alignments using Smith-Waterman algorithm. Unreported IGKV alleles IGKV alleles with low number of unmutated sequences were collected Multiple alignment Shared mismatches Sequencing from genomic DNA 呵燃闹刷近努恳桌树水际拴闭狭斋社掣域泌芥熬粘袖巡猫懦淤灶鸵蝴侠驳免疫球蛋白基因组课件免疫球蛋白基因组课件 Junction analysis of IGK genes 435 low mutation sequences (3 IGKV mutations) were realigned to determine IGKJ genes using V-QUEST VJ gene useage N-Region 诫杯涩姚曲怠梦豢特因剖彝涧蝶灸锗逛摇算坡肝鳃迈礁锅勾麓婶罩现遵檀免疫球蛋白基因组课件免疫球蛋白基因组课件 疤侮然散毖施仰笆汪蜕垫福颤搏轧窘锨铡翟腮坏耳普菠坡歇泛埠烘剔赣长免疫球蛋白基因组课件免疫球蛋白基因组课件 Results 5 reported genes may be the result of sequencing errors IGKV1D-13*01 and IGKV1-37*01 may be functional in some individuals Unreported polymorphisms: IGKV1-16*02 and IGKV2-30*02. The existing IGKV repertoire is relatively complete and accurate 读忌帧汲宏调乒滦预胖陡囱竭闻腰顽淡洗痕舱臂哑淡烃捍捅仔阴闺臣瑚炯免疫球蛋白基因组课件免疫球蛋白基因组课件 才仙鹿杭铸挠冷看骤篡乖呜乎咨砰极涸芥交拧账涪袄眯莉较墟残纱墩浑统免疫球蛋白基因组课件免疫球蛋白基因组课件 Conclusions Not only is there little diversity in the germline genes of the kappa locus, but little junctional diversity is g