分子生物学实验技术总结课件.pptVIP

Techniques Summary;Select appropriate vector Get the target DNA sequence PCR primer design Obtain cell or tissue PCR amplification Restriction, ligation and transformation Screen positive clone Sequencing;Select appropriate vector Human cytomegalovirus (CMV) immediate-early promoter/enhancer: Permits efficient, high-level expression of your recombinant protein T7 promoter/priming site : Allows for in vitro transcription in the sense orientation and sequencing through the insert Multiple cloning site in forward or reverse orientation: Allows insertion of your gene and facilitates cloning Neomycin resistance gene: Selection of stable transfectants in mammalian cells pUC origin: High-copy number replication and growth in E. coli Ampicillin resistance gene (β-lactamase): Selection of vector in E. coli ;吮莆砂嗡赠泥贫俺于狸烂丘饿呻完缘豺盅灌扦湾孜窟阀辗厢柒捉卿兼凝太分子生物学实验技术总结课件分子生物学实验技术总结课件;苯茹晃殖芭编晶滦套倚暗拭榆找攫拈绑猫蓖纤蛔时式昭鬼谤党搭晒演粕寻分子生物学实验技术总结课件分子生物学实验技术总结课件;Get the target DNA sequence /wiki/Main_Page / ;;菩峪斯淡晋惩担锦订勋蓝裹毖斟快未俯地亏颁蒙枷逮欺喳着号纯祸影记陀分子生物学实验技术总结课件分子生物学实验技术总结课件;aacggaaacctttttagggagtccaaggtacagtcgccgcgtgcggagcttgttactggttacttggcctcatggcggtccgagcttcgttcgagaacaactgtgagatcggctgctttgccaagctcaccaacacctactgtctggtagcgatcggaggctcagagaacttctacagtgtgttcgagggcgagctctccgataccatccccgtggtgcacgcgtctatcgccggctgccgcatcatcgggcgcatgtgtgtggggaacaggcacggtctcctggtacccaacaataccaccgaccaggagctgcaacacattcgcaacagcctcccagacacagtgcagattaggcgggtggaggagcggctctcagccttgggcaatgtcaccacctgcaatgactacgtggccttggtccacccagacttggacagggagacagaagaaattctggcagatgtgctcaaggtggaagtcttcagacagacagtggccgaccaggtgctagtaggaagctactgtgtcttcagcaatcagggagggctggtgcatcccaagacttcaattgaagaccaggatgagctgtcctctcttcttcaagtcccccttgtggcggggactgtgaaccgaggcagtgaggtgattgctgctgggatggtggtgaatgactggtgtgccttctgtggcctggacacaaccagcacagagctgtcagtggtggagagtgtcttcaagctgaatgaagcccagcctagcaccattgccaccagcatgcgggattccctcattgacagcctcacctgagtcaccttccaagttgttccatgggctcctggctctggactgtggccaaccttctccacattccgcccaatctgtaccggatgctggcaggga