基质金属蛋白酶9作用后大肠癌细胞肿瘤坏死因子受体1的变化.pdfVIP

山东医药2008年第48卷第l6期 基质金属蛋白酶9作用后大肠癌细胞 肿瘤坏死因子受体1的变化 彭 梅 。白 岚 (1长沙市第八医院，湖南长沙410001；2南方医科大学南方医院) [摘要】 目的 研究基质金属蛋白酶9(MMP-9)对大肠癌细胞肿瘤坏死因子受体1(TNFR1)表达的影响以及 通过促进其脱落形成可溶性肿瘤坏死因子受体1(sTNFR1)从而促进大肠癌侵袭和转移的可能途径。方法 免疫 荧光法研究TNFR1在大肠癌细胞SW1 1 16中的表达；用MMP-9干预培养的大肠癌SW1 1 16细胞，用流式细胞术检 测MMP-9不同剂量和不同作用时间时TNFR1表达变化，同时应用双抗体夹心ELISA法检测细胞培养上清中sTN— FR1的变化。结果 SW1116细胞表达TNFR1；MMP-9使SW1116细胞表面TNFR1脱落形成sTNFR1有剂量和时 间依赖性。结论 MMP一9促进大肠癌细胞表面TNFR1脱落形成sTNFR1，可能与大肠癌侵袭转移密切相关。 [关键词】 肠肿瘤；基质金属蛋白酶；肿瘤坏死因子受体1 [中图分类号】 R735．3 [文献标识码】 A [文章编号】 1002-266X(2008)16-0003-03 Change of tumor necrosis factor receptor-1 in human colon carcinoma celI Iines after effects of matrix metalloproteinase-9 PENGMei1 BAILan (1 Eighth Hosphal ofChangsha，Changsha 410001，P．R．China) Abstract：0bjective To study the effects of matrix metalloproteinase-9(MMP-9)on the expression of tumor necrosis factor receptor-1(TNFR1)and promotion effect on the process of sTNFR1 origlned from TNFR1 shedding from the cell sur- face which was correlated with migratory and invasive potentials in human colon carcinoma cells．Methods Immunofluo- rescence staining was applied to examine the expression of TNFR1 in SW1 l16 of human colon carcinoma cell lines．The e pression of TNFR1 Was assayed by flow e~ometry in human colon carcinoma SW1 1 16 cells treated with MMP-9 at different concentrations and at different time periods of culture．The change of sTNFR1 in cultured cell supernatant was measured by enzyme—linked immunosorbent assay(ELISA)．Results TNFR1 Was expressed in SW1 1 16 cells．MMP-9 encouraged the shedding of TNFR1 on cell surface to form sTNFR1 in a dose—dependent and time—dependent man／let．Conclusions MMP- 9 encouraged the shedding of TNFR1 on the Stlrface of human colon carcinoma cells to form sTNFR1 which may be a factor asseciated th invasive and metastasis potentials of colon carcinoma． Key words：intestinal neoplasms