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当代医学　2009年12月第15卷第36期总第191期　Contemporary Medicine, Dec. 2009,Vol.15 No.36 Issue No.191 基质金属蛋白酶-9mRNA在皮肤恶性黑素细胞瘤中的 表达及意义 李青 赵建斌 [摘要] 目的 研究基质金属蛋白酶-9(MMP-9)在皮肤恶性黑素细胞瘤中的表达及其意义。方法 采用逆转录-聚合酶链反应(RT-PCR)技术检测26例皮肤恶 性黑素细胞瘤、30例色素痣组织中MMP-9mRNA的表达。结果 19例皮肤恶性黑素细胞瘤组织中均有MMP-9mRNA的表达(74.1%)，5例色素痣组织中可见MMP-9mRNA的 P P 表达(15.6%)，两者相比差异具有统计学意义( ＜0.01)，有淋巴结转移皮肤黑色素瘤组MMP-9mRNA表达显著高于无淋巴结转移皮肤黑素瘤组( ＜0.05)。结论 MMP-9 在皮肤恶性黑色素细胞瘤表达增高，表明其在皮肤恶性黑色素瘤中的发生、发展及转移中可能起一定的作用，有望作为治疗皮肤恶性黑色素瘤的靶点。 [关键词] 恶性黑素瘤；MMP-9 [Abstract] Objective To investigate the expression of matrix metalloproteinase-9(MMP-9) mRNA in cutaneous melanoma. Methods The MMP-9 mRNA in twenty–six specimens of human cutaneous melanoma and thirty pigmented naevus were detected by semi-quantity reverse transcription-polymerase chain reaction(RT-PCR).Results MMP-9mRNA expression was detected in ninteen cutaneous melanoma(74.1%) and fi ve pigmented naevus(15.6%), there was signifi cant difference statistically between the two groups(P0.01).The expression of MMP-9mRNA in lymph node metastasis group was signifi cantly higher than non-lymph node metastasis group. Conclusion The expression of matrix metalloproteinases-9 is signifi cantly increased in cutaneous melanoma than that of pigmented naevus,it may play a important role in the pathogenesis of cutaneous melanoma. 论 Therefore,MMP-9 may have signifi cant therapeutic implications as molecular target. [Key words] Malignant melanoma; Matrix metalloproteinases-9 著 MMPs是一组锌离子依赖性肽链内切酶，其通过对细胞外基 1 资料与方法 质(ECM)中不同成份的降解，在肿瘤侵袭转移中起着关键性作用， 1.1 一般资料 选用2007～2009年我院门诊患者中收