提高病毒疫苗抗原产量的策略.PDFVIP

生 物 工 程 学 报 谢利豹 等/提高病毒疫苗抗原产量的策略 1213 Chinese Journal of Biotechnology /cjbcn August 25, 2017, 33(8): 1213−1223 DOI: 10.13345/j.cjb.160386 ©2017 Chin J Biotech, All rights reserved ·综 述· 谢利豹，李永锋，张玲楷，仇华吉 150069 , , , . . , 2017, 33(8): 1213−1223. Xie LB, Li YF, Zhang LK, et al. Strategies for producing high-yield viral vaccine antigens. Chin J Biotech, 2017, 33(8): 1213−1223. 摘 要: 疫苗接种是预防传染病的一种重要策略。然而，目前许多疫苗在生产过程中存在抗原产量偏低的问 题，由此导致疫苗的生产成本较高、有效抗原含量低和免疫效果差等问题。为此，研究人员尝试了不同策略来 提高病毒疫苗抗原的产量，以改进疫苗的质量并降低生产成本。文中总结了近年来提高疫苗中病毒抗原产量的 主要方法，包括改造病毒基因、改善病毒对细胞的适应性、优化抗原表达体系、改进疫苗生产工艺等方面。并 分析了不同策略的优点和存在的问题，提出了提高疫苗抗原产量的一些设想。 : 病毒疫苗抗原，疫苗生产，高产毒株 Strategies for producing high-yield viral vaccine antigens Libao Xie, Yongfeng Li, Lingkai Zhang, and Huaji Qiu Harbin Veterinary Research Institute, CAAS, Harbin 150069, Heilongjiang, China Abstract: Vaccination is an important strategy to prevent infectious diseases. However, low antigen yield of vaccine producing strains may lead to high cost of vaccines, low antigen production and vaccine failure. In recent years, many efforts have been made to improve the antigen yield of many vaccines. This mini-review summarizes various methods for increasing the antigen yield for vaccine production, including genetic modification of viruses, improvement of the adaptation of viruses to cells, and optimization of antigen expression systems and manufacturing procedures. Furthermore, we discuss the advantages and the problems of current strategies, as well as indicate the perspectives. Keywords: viral vaccine antigen, vaccine production, high-yield virus strain Received: October 17, 20